| 猪源大肠杆菌O157:H7体外氟苯尼考耐药诱导及对抗菌药物敏感性变化研究 |
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| 引用本文： | 冯世文,李军,曾芸,杨威,陈泽祥,潘艳,彭昊. 猪源大肠杆菌O157:H7体外氟苯尼考耐药诱导及对抗菌药物敏感性变化研究[J]. 中国畜牧兽医, 2015, 42(12): 3315-3322. DOI: 10.16431/j.cnki.1671-7236.2015.12.030 |
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| 作者姓名： | 冯世文 李军 曾芸 杨威 陈泽祥 潘艳 彭昊 |
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| 作者单位： | 1. 广西大学动物科学技术学院, 南宁 530005;2. 广西兽医研究所, 南宁 530001;3. 广西畜禽疫苗新技术重点实验室, 南宁 530001 |
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| 基金项目： | 广西自然科学基金(桂科自2013GXNSFAA019093);广西水产畜牧兽医局科技项目(桂渔牧科201528005);广西畜禽疫苗新技术重点实验室专项(13-051-27-A-3、14-045-31-B-4) |
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| 摘 要： | 为初步研究猪源大肠杆菌O157:H7 (E.coli O157:H7)对氟苯尼考耐药性的产生和消除机制,本研究采用亚抑菌浓度体外耐药诱导的方法将两株猪源大肠杆菌O157:H7诱导成氟苯尼考高度耐药菌株,采用无氟苯尼考压力下连续传代培养的方法将获得的氟苯尼考耐药菌株的氟苯尼考耐药性消除,检测耐药诱导菌和耐药消除菌对抗菌药物的敏感性,并检测菌株质粒携带的耐药基因。结果显示,经氟苯尼考耐药诱导,猪源大肠杆菌O157:H7对氟苯尼考、阿莫西林、头孢唑啉、头孢拉定和头孢噻吩由敏感变为耐药,对头孢噻肟的敏感性由敏感变为中介,对氧氟沙星、环丙沙星和阿奇霉素由中介变为耐药;而经耐药消除后,菌株恢复对上述药物的敏感性;在菌株的质粒中检测到氟苯尼考耐药基因、喹诺酮类耐药基因和β-内酰胺酶基因,与耐药表型相符。结果表明,在氟苯尼考压力的长期存在下,猪源大肠杆菌O157:H7对氟苯尼考产生耐药,且对青霉素类、头孢类和喹诺酮类药物产生交叉耐药,在去除氟苯尼考压力下连续培养,可消除菌株的部分耐药性。
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| 关 键 词： | 猪源大肠杆菌O157:H7 氟苯尼考 耐药诱导 耐药消除 交叉耐药 |
| 收稿时间： | 2015-07-22 |
Study on Florfenicol-resistant Induction in vitro and Changes of Drug Sensitivity of Swine E.coli O157:H7 |
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| FENG Shi-wen,LI Jun,ZENG Yun,YANG Wei,CHEN Ze-xiang,PAN Yan,PENG Hao. Study on Florfenicol-resistant Induction in vitro and Changes of Drug Sensitivity of Swine E.coli O157:H7[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(12): 3315-3322. DOI: 10.16431/j.cnki.1671-7236.2015.12.030 |
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| Authors: | FENG Shi-wen LI Jun ZENG Yun YANG Wei CHEN Ze-xiang PAN Yan PENG Hao |
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| Affiliation: | 1. College of Animal Science and Technology, Guangxi University, Nanning 530005, China;2. Guangxi Veterinary Institute, Nanning 530001, China;3. Guangxi Key Laboratory of Animal Vaccines and New Technology, Nanning 530001, China |
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| Abstract: | To preliminary investigate the mechanism of drug resistance and drug-resistant elimination of swine E.coli O157:H7,sub-inhibitory concentration in vitro drug-induced method was used in this study,for inducing two strains of E.coli O157:H7 becoming florfenicol highly resistant strains,and eliminated florfenicol resistance of florfenicol-resistant strains by continuous cultured without florfenicol pressure.The drug sensitivity and resistant genes of florfenicol-induced strains and florfenicol-resistance eliminated strains were detected.The results showed that compared with original strains,after drug-resistant induction by florfenicol,swine E.coli O157:H7 were resistant to florfenicol,amoxicillin,cefazolin,cephradine and cephalothin from high sensitivity,and resistant to cefotaxime,ofloxacin,ciprofloxacin and azithromycin from moderate sensitivity.However,after florfenicol-resistance eliminated,swine E.coli O157:H7 restored sensitivity to these drugs.In addition,florfenicol resistance gene,quinolone resistance genes and β-lactamase genes were found in the plasmids of swine E.coli O157:H7,the resistance genes were consistent with the resistance phenotype.According to the results,with the long-term presence of florfenicol pressure,swine E.coli O157:H7 resistant to florfenicol,and acquired penicillins,cephalosporins and quinolones cross-resistance;While swine E.coli O157:H7 was eliminated partial drug-resistance by continuous culture without florfenicol pressure. |
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| Keywords: | swine E.coli O157:H7 florfenicol drug-resistant induction drug-resistant elimination cross-resistance |
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