Study on Effect of Nitrogen and Phosphorous on the Resistance of E.coli to Chloramphenicol and its Mechanism |
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Authors: | HUANG Qi-fa CHEN Xiu-ping LIN Jing ZHANG Jie WANG Yan-qiu YU Dao-jin |
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Institution: | College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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Abstract: | This study was designed to explore the effect of nitrogen and phosphorous on the resistance of E.coli from environment and the mechanism.Microcosms were established to study the effect of nitrogen and phosphorous on the resistant phenotype of E.coli to chloramphenicol (CHL).cat gene of isolated drug-resistant strains and susceptible strains were detected.The results showed that,different concentration of nitrogen and phosphorous could induce the formation of antibiotics resistance of E.coli to CHL.The rate of cat gene of 46 strains of chloramphenicol resistant E.coli was 89.13%,which was 0 in the 16 strains of chloramphenicol sensitive E.coli.The results indicated that,nitrogen and phosphorous in the microcosms could induce the formation and maintenance of resistance to chloramphenicol in E.coli,which had correlation with cat gene. |
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Keywords: | E coli microcosm nitrogen and phosphorus resistance cat gene |
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