胡萝卜品种资源遗传多样性及亲缘关系研究

结合表型和SSR标记对29份中国胡萝卜地方资源、40份橘色栽培品种以及1份土耳其紫色品种进行遗传多样性和亲缘关系研究。田间农艺性状多样性分析表明,除叶长、根长、根粗外,春秋两季胡萝卜橘色栽培品种的多样性指数平均值低于地方资源。主成分分析结果表明,中国橘色地方资源和橘色栽培品种表型在秋季较为相似,但在春季差异较大。利用38对SSR引物对胡萝卜品种进行扩增,共扩增出133个多态性位点,其中中国地方资源与橘色栽培品种的平均多态性位点数分别为4.2和2.9个。中国地方资源的多态性信息含量值(0.74±0.06)明显高于橘色栽培品种(0.42±0.03)。聚类分析将供试胡萝卜材料分为2组,第I组包括所有橘色栽培品种和橘色地方资源B0241,第Ⅱ组包含其他地方资源和紫色品种L1。

Studies on Genetic Diversity and Relationship of Carrot Varieties and Germplasm Resources

Genetic diversity and relationship between 29 Chinese carrot landraces，40 orange varieties and 1 Turkey purple cultivar were investigated using phenotypic and SSR markers. Phenotypic result showed that diversity index of orange varieties was lower than Chinese landraces except for leaf length，root length and root diameter. Principal component analysis showed that Chinese orange landraces had similar phenotypic variation with orange varieties in the autumn，but different in the spring. 133 polymorphic loci were amplified from 38 pairs of SSR primers. The average number of polymorphic loci of Chinese carrot landraces and orange varieties were 4.2 and 2.9，respectively. PIC value of Chinese carrot landraces（0.74±0.06）was obviously higher than that of orangevarieties（0.42±0.03）. All accessions were divided into 2 groups: group I including orange varieties and orange B0241，and group Ⅱ containing other landraces and purple cultivar L1.