Determination of the Complete Genome of DHAV GX Strain and Bioinformatics Analysis of VP1 Protein

In order to investigate the genotypic characteristics and biological characteristics of major capsid protein (VP1) of duck hepatitis A virus (DHAV) GX strain, the whole genome of GX strain was sequenced and compared with 3 serotypes reference strains of DHAV by the molecular biology software.The results showed that the full-length genome of DHAV GX strain was 7 800 bp, including 5'UTR (652 bp), 3'UTR (369 bp) and ORF (6 756 bp) encoding 2 251 aa and its coding products were 12 (VP0/VP3/VP1/2A1/2A2/2A3/2B/2C/3A/3B/3C/3D) at least.Sequence analysis showed that DHAV GX strain could be ranked DHAV 3 style.This strain shared the highest homology with DHAV-3 reference strains; Furthermore, GX strain and DHAV-3 FS strain were in the same cluster, might come from the same ancestor.Amino acid segments locating in 195 to 201 and 211 to 221 aa could be larvaceous dominant B-cell linear epitopes.It suggested that VP1 gene could be used as a candidate gene for the development of genetic engineering vaccine of DHAV.