Radix Pseudostellariae polysaccharide attenuates high fat diet induced hepatic insulin resistance in mice |
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Authors: | WANG Qi CHAI Dan-dan WU Xiao-hua REN Li-we LIU Yong-nian YU Zhi-wen |
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Institution: | 1. Department of Pathophysiology, Medical College of Qinghai University, Xining 810000, China;
2. Department of Research and Development, Fujian Mindong Rejuvenation Pharmaceutical Co., Ltd, Ningde 352000, China;
3. Biological Medicine Research and Development Center, Fujian Traditional Chinese Medicine University, Fuzhou 350000, China |
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Abstract: | AIM: To study the role of Radix Pseudostellariae polysaccharide (RPP) in hepatic insulin resistance.METHODS: Six-week-old C57BL/6J mice were randomly divided into low-fat diet (LFD) control group and high-fat diet (HFD) model group. After 16 weeks, intraperitoneal pyruvate tolerance test (IPPTT) was performed to determine the establishment of the HFD-induced hepatic insulin resistance model. HFD containing RPP (500 mg/kg) was given for 4 consecutive weeks. IPPTT, liver malondialdehyde (MDA) level and liver mitochondrial MDA level were measured. The protein levels of p-AKT (Ser473/Thr308), p-AMPK, nuclear factor E2-related factor 2 (Nrf2), NQO1 and IκBα in the liver tissues were measured by Western blot.RESULTS: After administration of RPP, a significant reduction in the levels of blood glucose and hepatic mitochondrial MDA was observed. The levels of p-AKT (Ser473/Thr308) and p-AMPK were significantly elevated in the liver tissues. The hepatic IκBα levels were up-regulated. RPP also enhanced the expression of Nrf2 system-regulated proteins NQO1 and HO-1 in the liver tissues.CONCLUSION: Radix Pseudostellariae polysaccharides effectively reduce HFD-induced hepatic insulin resistance in C57BL/6J mice and improves liver glucose metabolism by ameliorating HFD-impaired hepatic transduction of insulin signaling, activating Nrf2-associated signaling and inhibiting the expression of inflammatory signaling proteins. |
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Keywords: | C57BL/6J mice Insulin resistance Radix Pseudostellariae polysaccharide Oxidative stress Malondialdehyde |
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