Protective effect of sodium selenite on human keratinocytes from ultraviolet-B irradiation |
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Authors: | LIU Sai-jun GUO Mei-yan DENG Lie-hua ZHAO Gang HU Yun-feng |
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Institution: | 1. Department of Dermatology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China;
2. Affiliated Hospital of Hebei University of Engineering, Handan 056002, China |
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Abstract: | AIM: To investigate the protective effect of sodium selenite (Na2SeO3) on human keratinocytes under ultraviolet-B (UVB) irradiation. METHODS: The cultured HaCaT cells were divided into 4 groups: (1) normal control group; (2) Na2SeO3 group: pretreated with Na2SeO3 at doses of 10 nmol/L, 50 nmol/L, 100 nmol/L, 200 nmol/L and 1 μmol/L for 24 h; (3) UVB group: irradiated with UVB at doses of 300, 600 and 900 J/m2 ; (4) Na2SeO3+UVB group: after pretreated with Na2SeO3 for 24 h, irradiated with UVB at doses of 300, 600 and 900 J/m2 . The cell proliferation was detected by MTT assay. The apoptotic rates of HaCaT cells treated with UVB at dose of 300 J/m2 were assessed by flow cytometry. RESULTS: Compared with normal control group, the cell proliferation activity in UVB group decreased significantly (P<0.05). The cell activity was inversely correlated with the irradiation intensity. No significant difference of the cell activity between Na2SeO3 group and normal control group was observed. The cell proliferation in Na2SeO3+UVB group was higher than that in UVB group significantly (P<0.05). Na2SeO3 at concentration of 100 nmol/L showed the strongest activity to promote cell proliferation. After 300 J/m2 UVB irradiation, the apoptotic rate in Na2SeO3+UVB group decreased significantly (P<0.05) compared with UVB group. The inhibitory effect of Na2SeO3 at concentration of 100 nmol/L on apoptosis was the strongest.CONCLUSION: The damage of human keratinocytes by UVB irradiation is in a dose-dependent manner. The photoprotection performance of Na2SeO3 reduces the damage of human keratinocytes induced by UVB irradiation. |
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Keywords: | Ultraviolet rays Keratinocytes Photoprotection Sodium selenite |
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