不同类型花椰菜硫代葡萄糖苷组分与含量分析

采用HPLC法测定了3种类型15个花椰菜品种的硫代葡萄糖苷(Glucosinolates,简称硫苷)组分及含量。结果表明:供试花椰菜品种均含有9种硫苷,包括5种脂肪族硫苷:3-甲基硫氧烯丙基硫苷(Glucoiberin,IBE)、2-羟基-3-丁烯基硫苷(Progoitrin,PRO)、2-丙烯基硫苷(Sinigrin,SIN)、4-甲基硫氧丁基硫苷(Glucoraphanin,RAA)和3-丁烯基硫苷(Gluconapin,NAP);4种吲哚族硫苷:4-羟基吲哚基-3-甲基硫苷(4-Hydroxyglucobrassicin,4OH)、3-甲基吲哚基硫苷(Glucobrassicin,GBC)、4-甲氧基吲哚基-3-甲基硫苷(4-Methoxyglucobrassicin,4ME)和1-甲氧基吲哚基-3-甲基硫苷(Neoglucobrassicin,NEO)。各品种的吲哚族硫苷总含量均高于脂肪族硫苷总含量,其中NEO和GBC是花椰菜的主要硫苷组分,分别占总硫苷含量的42.61%和35.02%。不同花椰菜品种的硫苷总含量差异较大,变异范围在0.3323~4.8728μmol·g-1(FW)之间;紫花菜的总硫苷含量最高,松花菜其次,紧花菜最低。

Analysis of Glucosinolate Components and Contents in Different Cauliflower Types

Glucosinolate components and contents in 15 cauliflower（Brassica oleracea L. var. botrytisL.）varieties from 3 types，were tested by HPLC method．The result indicated that 9 different components of glucosinolate were detected，including 5 aliphatic glucosinolates（Glucoiberin，Progoitrin，Sinigrin，Glucoraphanin，Gluconapin），and 4 indolic glucosinolates（4-Hydroxyglucobrassicin，Glucobrassicin，4-Methoxyglucobrassicin，Neoglucobrassicin）．The indolic glucosinolates contents of each variety were higher than that of aliphatic glucosinolate．Among them，the major glucosinolate contents of cauliflower Neoglucobrassicin and Glucobrassicin took 42.61% and 35.02% of the total glucosinolates，respectively．Therewere big differences in total glucosinolates content of different cauliflower varieties．The variation scope was between 0.332 3-4.872 8 μmol·g^-1（FW）．The total glucosinolates content of purple-head cauliflower was the highest，loosen-head cauliflower took the second place，and compact-head cauliflower was the least．