Tanshinone IIA prevents high glucose-induced human umbilical vein endothelial cell apoptosis |
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Authors: | YU Jian-xin WU Qi YANG Huan |
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Institution: | Department of Endocrinology, Affiliated Hospital of Jiujiang University, Jiujiang 332000, China |
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Abstract: | AIM: To investigate the effect of tanshinone IIA on the apoptosis of human umbilical vein endothelial cells(HUVECs) after high glucose treatment.METHODS: The cell viability was determined by MTT assay. The cell apoptotic rate was examined by flow cytometry with Annexin V-FITC/PI double staining. The expression of Bcl-2 and Bax, and the release of mitochondrial cytochrome C(Cyt C) were analyzed by Western blotting.RESULTS: Tanshinone IIA significantly inhibited high glucose-induced decrease in cell viability and increased the cell apoptosis. Additionally, after tanshinone IIA treatment, Bax expression and the release of mitochondrial Cyt C were significantly inhibited, while Bcl-2 expression was increased.CONCLUSION: Tanshinone IIA prevents high glucose-induced endothelial cell apoptosis via mitochondria-dependent pathway. |
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Keywords: | Tanshinone IIA High glucose Endothelial cells Apoptosis |
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