Cloning and Expression Pattern Analysis of KAP8.2 Gene in Sheep

The object of this study was to clone the Keratin-associated protein 8.2 (KAP8.2) gene from Small-tail Han sheep and Xinji Fine-wool sheep and test the tissues expression pattern.In this study,the total RNA were extracted from different tissues firstly,and KAP8.2 gene was cloned by RT-PCR.In the end,the different expression patterns of KAP8.2 gene in Small-tail Han sheep and Xinji Fine-wool sheep was detected by QRT-PCR.The cloning results showed that the KAP8.2 gene were cloned,and the length of KAP8.2 mRNA was 574 bp,containing a 192 bp ORF which encoded a protein of 63 amino acids.These KAP8.2 gene belonged the typical HGT-KAP family because the rate of glycine and tyrosine were 23.8% and 20.6%,respectively.The polymorphism analysis showed that there were some polymorphisms in KAP8.2 gene and almost SNPs located on both sides of CDS region.The region which located on the 3'UTR c192+19-39 of KAP8.2 from Small-tail Han sheep might be the target of fru-let-7j.The expression pattern analysis showed that KAP8.2 gene was a multiple organs expression gene,and there were significant differences in expression patterns of KAP8.2 gene in Small-tail Han and Xinji Fine-wool sheeps.In Small-tail Han sheep,KAP8.2 gene was over-expressed in spleen,liver and skin,but in Xinji Fine-wool sheep,KAP8.2 gene was over-expressed in skin and heart.In conclusion,there were significant differences in gene polymorphisms and expression pattern between Small-tail Han sheep and Xinji Fine-wool sheep,and the result suggested the KAP8.2 gene associated with some phenotypes of wool.