Chemical Characterization of Atlantic Cod (Gadus morhua) Collagen Hydrolyzed Using Enzyme Preparation Derived from Red King Crab (Paralithodes camtschaticus) and Its Potential as a Core Component of Bacterial Culture Medium |
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| Authors: | Vitaliy Yu Novikov Nadezhda V Shumskaya Vyacheslav A Mukhin Konstantin V Zolotarev Anton N Mikhailov Valeriya I Nakhod Marina V Mikhailova |
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| Institution: | 1.Polar Branch, Russian Federal Research Institute of Fisheries and Oceanography, 6 Akademik Knipovich Street, 183038 Murmansk, Russia; (V.Y.N.); (N.V.S.); (V.A.M.);2.Laboratory of Environmental Biotechnology, Institute of Biomedical Chemistry, 10 Pogodiskaya Street, 119121 Moscow, Russia; (A.N.M.); (V.I.N.); (M.V.M.) |
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| Abstract: | The Atlantic cod (Gadus morhua) and red king crab (Paralithodes camtschaticus) processing wastes are massive and unutilized in the Murmansk region of Russia. The samples of skin-containing waste of Atlantic cod fillets production were hydrolyzed using enzyme preparations derived from red king crab hepatopancreases, porcine pancreases, and Bacillus subtilis bacteria. The activity of enzymes from crab hepatopancreases was significantly higher than the activity of enzymes derived from other sources. The optimal conditions of the hydrolysis process have been figured out. The samples of cod processing waste hydrolysate were analyzed for amino acid composition and molecular weight distribution. The samples of hydrolysate were used as core components for bacterial culture medium samples. The efficiency of the medium samples was tested for Escherichia coli growth rate; the most efficient sample had an efficiency of 95.3% of that of a commercially available medium based on fish meal. Substitution of medium components with those derived from industrial by-products is one of the ways to decrease a cost of a culture medium in biopharmaceutical drug production. |
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| Keywords: | processing waste Atlantic cod collagen red king crab enzyme preparation collagen hydrolysate culture medium |
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