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铁皮石斛组培育苗与组培苗培植技术研究
引用本文:罗万业,罗万周,魏锦秋,陈桂琼,朱昔娇. 铁皮石斛组培育苗与组培苗培植技术研究[J]. 广东林业科技, 2014, 30(2): 78-81
作者姓名:罗万业  罗万周  魏锦秋  陈桂琼  朱昔娇
作者单位:广东省梅州市林业科学研究所,广东省梅州市大埔县高陂镇林业站,广东省梅州市林业科学研究所,广东省梅州市林业科学研究所,广东省梅州市林业科学研究所
摘    要:
对铁皮石斛组织培养育苗和组培苗培植技术进行研究,结果表明:在MS培养基中添加6-BA 1.0mg/L+NAA 0.1 mg/L+IAA 0.5 mg/L+50 g/L椰子汁有利于芽苗的增殖;在1/2MS培养基中添加6-BA0.1 mg/L+IBA 1.0 mg/L+NAA 0.2 mg/L+50 g/L椰子汁有利于小苗生根和植株生长。搭建大棚移植铁皮石斛组培苗,采用高架苗床,选用松树皮和花生壳(v/v=4∶1)为基质,移栽成活率可达95%以上。

关 键 词:铁皮石斛  组织培养  培植技术
收稿时间:2014-03-02
修稿时间:2014-03-24

Study on the Techniques of Tissue Culture and Cultivation of Dendrobium officinale
Luo Wanye,Luo Wanzhou,Wei Jinqiu,Chen Guiqiong and zhuxijiao. Study on the Techniques of Tissue Culture and Cultivation of Dendrobium officinale[J]. Forestry Science and Technology of Guangdong Province, 2014, 30(2): 78-81
Authors:Luo Wanye  Luo Wanzhou  Wei Jinqiu  Chen Guiqiong  zhuxijiao
Affiliation:Guangdong Province Meizhou City Forestry Science Institute,Guangdong Meizhou,Guangdong Province Meizhou City Forestry Science Institute,Guangdong Meizhou,
Abstract:
The tissue culture and cultivation techniques of Dendrobium officinale were studied, the results showed that: the MS medium supplemented with 6-BA 1.0 mg/L NAA 0.1 mg/L IAA 0.5 mg/L 50 g/L coconut juice was good for bud proliferation; the 1/2MS medium supplemented with 6-BA 0.1 mg/ L IBA 1.0 mg/L NAA 0.2 mg/L 50 g/L coconut juice was good for rooting and growth of seedlings.The transplantion survival rate could reach above 95%,when the plantlets of D. officinale were planted under the conditions of greenhouse, elevated seedbed and growing media with pine bark peanut shell (v/v =4:1).
Keywords:Dendrobium officinale   tissue culture   cultivation techniques
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