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| 油桃菌核病菌原分子检测 | |
| 引用本文： | 周增强,王丽,侯珲,苏平,田娟. 油桃菌核病菌原分子检测[J]. 果树学报, 2012, 0(2): 283-285,321 |
| 作者姓名： | 周增强 王丽 侯珲 苏平 田娟 |
| 作者单位： | 中国农业科学院郑州果树研究所;安徽省砀山园艺场 |
| 基金项目： | “十一五”国家科技支撑计划重点项目(2006BAD07B02) |
| 摘 要： | 通过病原菌形态学和致病性测定的方法对引起设施油桃花腐的病原菌进行了分离鉴定,采用PCR技术扩增病菌DNA-ITS基因,序列测定结果表明该片段序列与GenBank已有的Sclerotinia sclerotiorum序列的同源性达到100%。综合2种方法鉴定结果,Sclerotinia sclerotiorum是引起设施油桃菌核病的致病菌。 |
| 关 键 词： | 油桃菌核病 形态学 致病性 rDNA-ITS测序 |
Molecular detection of Sclerotinia sclerotiorum from nectarine |
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| ZHOU Zeng-qiang,WANG Li,HOU Hui,SU Ping,TIAN Juan. Molecular detection of Sclerotinia sclerotiorum from nectarine[J]. Journal of Fruit Science, 2012, 0(2): 283-285,321 | |
| Authors: | ZHOU Zeng-qiang WANG Li HOU Hui SU Ping TIAN Juan |
| Affiliation: | 1Zhengzhou Fruit Research Institute of CAAS,Zhengzhou,Henan 45009 China;2Anhui Province Dangshan Horticultural Field,Dangshan,Anhui 235300 China) |
| Abstract: | A pathogenic fungus causing flower rot of greenhouse-grown nectarine was isolated and characterized based on the pathogenic morphology and pathogenicity.By using polymerase chain reaction(PCR)technique to amplify rDNA-ITS of the isolate,the results revealed that rDNA-ITS sequence of the isolate and Sclerotinia sclerotiorum shared 100% homology,which was compared in NCBI GenBank.Synthesized two identification results indicate that Sclerotinia sclerotiorum was the pathogen causing nectarine sclerotinia. |
| Keywords: | Nectarine Sclerotinia blight Pathogenic morphology Pathogenicity rDNA-ITS sequencing |
| 本文献已被 CNKI 等数据库收录！ | |