| 马尾藻多糖对鸡脾脏淋巴细胞增殖及氧化应激影响的实验观察 |
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| 引用本文： | 张玲,胡庭俊,陈炳全.马尾藻多糖对鸡脾脏淋巴细胞增殖及氧化应激影响的实验观察[J].西南农业学报,2011,24(5). |
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| 作者姓名： | 张玲 胡庭俊 陈炳全 |
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| 作者单位： | 广西大学动物科学技术学院,广西南宁,530005 |
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| 基金项目： | 国家自然科学基金项目，广西科学研究与技术开发计划项目 |
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| 摘 要： | 马尾藻具有很高的食用和药用价值,分离提取马尾藻多糖(Sargassum polysaccharides,SP),对其理化性质进行分析；研究马尾藻多糖对氧化应激态鸡脾脏淋巴细胞内谷胱甘肽(GSH)和一氧化氮(NO)分泌水平及淋巴细胞体外增殖的影响.结果表明:马尾藻多糖提取率为152.8 g/L;马尾藻多糖浓度为100、200、400 mg/L时,能显著促进体外培养的鸡脾脏淋巴细胞增殖；H2O2浓度为50 μmol/L时,能诱导体外培养的鸡脾脏淋巴细胞处于氧化应激状态.马尾藻多糖浓度为400 mg/L,与细胞共孵育4h或12h,能显著增加氧化应激态细胞内GSH含量；共孵育培养8h,能显著降低氧化应激态细胞的NO水平.马尾藻多糖能促进鸡脾脏淋巴细胞增殖,并通过调节细胞内GSH和NO水平,提高免疫细胞的抗氧化能力,发挥免疫调节作用.
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| 关 键 词： | 马尾藻多糖 鸡 脾脏淋巴细胞 增殖 氧化应激 免疫 |
Effects of Sargassum polysaccharide on Proliferation and Oxidative Stress of Splenic Lymphocytes in Chicken |
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| ZHANG Ling,HU Ting-jun,CHEN Bing-quan.Effects of Sargassum polysaccharide on Proliferation and Oxidative Stress of Splenic Lymphocytes in Chicken[J].Southwest China Journal of Agricultural Sciences,2011,24(5). |
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| Authors: | ZHANG Ling HU Ting-jun CHEN Bing-quan |
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| Institution: | ZHANG Ling,HU Ting-jun,CHEN Bing-quan (College of Animal Science and Technology,Guangxi University,Guangxi Nanning 530005,China) |
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| Abstract: | The isolation and purification of Sargassum polysaccharides was carried out and the physicochemical property of Sargassum polysaccharide was analyzed.The effects of S.polysaccharide on glutathione concentration,nitric oxide production and cell proliferation were investigated in vitro.The results showed that S.polysaccharide was obtained with a yield of 152.8 g/L(w/w).S.polysaccharide at concentration of 100,200 or 400 mg/L could significantly enhance the lymphocyte proliferation(P<0.05).Hydrogen peroxide at... |
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| Keywords: | Sargassum polysaccharides Chicken Oxidative stress Immunity |
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