| 广西巴马小型猪成纤维细胞体外培养体系的建立 |
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| 引用本文： | 王献伟,卢晟盛,卢克焕.广西巴马小型猪成纤维细胞体外培养体系的建立[J].西南农业学报,2011,24(5). |
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| 作者姓名： | 王献伟 卢晟盛 卢克焕 |
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| 作者单位： | 亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004;广西大学动物繁殖研究所,广西南宁530004 |
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| 基金项目： | 国家自然科学基金资助项目，农业都"转基因生物新品种培育重大专项"项目，广西自然科学基金重点项目，广西大学拔尖创新团队建设计划项目 |
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| 摘 要： | 为建立广西巴马小型猪腹部成纤维细胞、耳成纤维细胞、睾丸成纤维细胞、肺部成纤维细胞及脾成纤维细胞体外培养体系,以新生巴马小型猪为试验材料,使用微量液体组织块贴壁法原代培养分离几种成纤维细胞,进行常规传代培养、冷冻和复苏；根据细胞的形态、生长特性等观察其体外生长能力和增殖寿命.结果显示,广西巴马小型猪腹部成纤维细胞、耳成纤维细胞、睾丸成纤维细胞、肺部成纤维细胞及脾成纤维细胞获得成功分离,体外可以传代和冷冻；冷冻复苏后仍可以正常培养和传代；腹部成纤维细胞免疫荧光检测结果显示波形蛋白表达阳性,角形蛋白表达阴性.研究成果可为显微操作核移植、手工核移植和转基因相关操作等提供不同类型的供体材料.
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| 关 键 词： | 广西巴马小型猪 成纤维细胞 细胞体外培养 |
Establishment of Vitro Culture System for Guangxi Bama Mini-pigs Fibroblast |
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| WANG Xian-wei,LU Sheng-sheng,LU Ke-huan.Establishment of Vitro Culture System for Guangxi Bama Mini-pigs Fibroblast[J].Southwest China Journal of Agricultural Sciences,2011,24(5). |
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| Authors: | WANG Xian-wei LU Sheng-sheng LU Ke-huan |
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| Institution: | WANG Xian-wei1,2,LU Sheng-sheng1,LU Ke-huan1,2 (1.National Key Laboratory for Conservation and Utilization of Subtropical Agro-Biotic Resources,Guangxi University,Guangxi Nanning 530004,China,2.Animal Reproduction Institute,China) |
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| Abstract: | To establish in vitro culture system of abdominal fibroblast,ear fibroblast,testicular fibroblast,pulmonary fibroblast and splenic fibroblast,the newborn Bama mini pigs were taken as material,micro-liquid tissue block adhering wall method was used for primary culture and isolation of the above cells,routine subculture,freezing,and recovery procedure were performed;and their growth capacity and proliferation life span were observed in vitro according to cell morphology and growth characteristics.The results ... |
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| Keywords: | Guangxi Bama mini-pigs Fibroblast Cell in vitro culture |
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