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| NCM-ELISA检测马铃薯Y病毒(PVY)技术的研究及应用 | |
| 引用本文： | 吕典秋,李学湛,吕文河,于德才,李辉. NCM-ELISA检测马铃薯Y病毒(PVY)技术的研究及应用[J]. 中国马铃薯, 2006, 20(6): 339-342 |
| 作者姓名： | 吕典秋 李学湛 吕文河 于德才 李辉 |
| 作者单位： | 1. 黑龙江省农业科学院植物脱毒苗木研究所,黑龙江,哈尔滨,150086;东北农业大学,黑龙江,哈尔滨,150030 2. 黑龙江省农业科学院植物脱毒苗木研究所,黑龙江,哈尔滨,150086 3. 东北农业大学,黑龙江,哈尔滨,150030 4. 黑龙江省农业科学院谷物品质研究中心,黑龙江,哈尔滨,150086 |
| 摘 要： | 血清学方法是病毒检测的主要手段。本试验通过对马铃薯Y病毒(PVY)的提纯,免疫家兔制备PVY抗血清,并提取PYV免疫球蛋白IgG作为NCM-ELISA反应为一抗,以市售羊抗兔抗血清为二抗,在硝酸纤维素膜(NCM)上进行NCM-ELISA反应检测PVY,建立PVY NCM-ELISA检测反应体系。试验结果显示,NCM-ELISA具有反应特异性强,灵敏度高的优点,检测植物叶片样品的最高稀释度可达到1:250。通过对田间40份样品的NCM-ELISA和DAS-ELISA检测比较,其检测结果吻合率达到100%。由于NCM-ELISA方法可以将样品点在硝酸纤维素膜上,并且可贮存几个星期或将膜送到其他实验室进行检测,因此具有操作简单,使用方便,检测成本低等优点。 |
| 关 键 词： | 硝酸纤维素膜-酶联免疫分析 马铃薯Y病毒 检测 |
| 文章编号： | 1672-3635（2006）06-0339-04 |
| 收稿时间： | 2006-03-08 |
| 修稿时间： | 2006-03-08 |
Detection of Potato Virus Y by NCM-ELISA Method |
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| Lu Dianqiu,Li Xuezhan,Lu Wenhe,Yu Decai,Li Hui. Detection of Potato Virus Y by NCM-ELISA Method[J]. Chinese Potato, 2006, 20(6): 339-342 | |
| Authors: | Lu Dianqiu Li Xuezhan Lu Wenhe Yu Decai Li Hui |
| Affiliation: | 1. Viruses-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang 150086, China; 2. Northeast Agricultural University, Harbin, Heilongjiang 150030, China; 3. Crop Quality Research Center, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang 150086, China |
| Abstract: | Serological technique as important method for viruse diagnose has been applied extensively in the field of disease detection.In this research,potato virus Y(PVY) was purified and the rabbit was immunized withthe purified virus of PVY for antiserum preparation.IgG of PVY was prepared as the fist antibody of NCM-ELISA and the commercial antiserum of goat-anti-rabbit was used as the second antibody.The reactions of ELISA were performed on NCM and the formal reaction system was established.The results showed that NCM-ELISA had the same merits of high specificity and sensitivity as DAS-ELISA.The highest detectable dilutions of extract of infected potato leaves was 1:250.NCM-ELISA had 100% consistency with DAS-ELISA based on comparison of test results from 40 potato samples collected from field.The samples can be added onto the NCM and the membrane can be stored for a few weeks or sent to other labs where the test can be done,so NCM-ELISA has the advantages of easy operation,convenience and low detection cost. |
| Keywords: | NCM-ELISA potato virus Y detection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |