15个西兰花芽苗菜中萝卜硫素含量差异研究

西兰花芽苗菜营养价值高且富含萝卜硫素，具有预防和抑制癌症的功效。为分析种质资源间萝卜硫素含量的差异，采用响应面中心复合设计法对超声浸提时间、水解时间和提取液pH条件3个因素进行优化分析，获得最优萝卜硫素提取方法，并用该方法对种质资源中萝卜硫素含量差异分析。结果表明，超声处理时间为12 min、pH条件为5.0、水解时间为4.3 h是提取西兰花芽苗菜萝卜硫素的最佳条件。利用该方法检测到15个种质资源的芽苗菜中萝卜硫素含量差异显著，其中CDBY-10中含量最高(506.896 μg/g)、CDBY-22中含量最低(50.416 μg/g)。15个种质资源中萝卜硫素含量主要分布在0~200 μg/g，频次拟合不显著。多数种质资源萝卜硫素含量符合晚熟>早熟>中熟的规律。CDBY-10、CDBY-20、CDBY-15可作为高萝卜硫素西兰花芽苗菜进行深入科学研究和商业开发。

Variety of Sulforaphane Content Among 15 Broccoli Sprouts

High nutrition, rich in sulforaphane, could prevent and inhibit cancer make broccoli sprouts becoming popular in foreign. Composite design of response surface analysis was taken to optimize the extraction of sulforaphane from broccoli sprouts. Then, the optimization extraction method was used to analysis variation of sulforaphane content among 15 broccoli sprouts. The result showed that optimization of extraction method was transonic processing time of 12 min, pH value of 5.0 and hydrolysis time of 4.3 h. There was a significant deference in content of sulforaphane among 15 germplasms of broccoli sprouts. The highest one was CDBY-10 (506.896 μg/g) and the lowest one was CDBY-22 (50.416 μg/g). Most of their contents were between 0-200 μg/g, frequency fitting were not well. Anymore, sulforaphane content in most of the germplasms was in line with late-maturing>early-maturing>mid-maturing. CDBY-10, CDBY-20, CDBY-15 need to be further studied and business developed as high sulforaphane content cultivars.