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| 生防细菌1505的鉴定及其对采后柑橘炭疽病的抑制效果 | |
| 引用本文： | 汪茜,胡春锦,黄思良,柯仿钢.生防细菌1505的鉴定及其对采后柑橘炭疽病的抑制效果[J].西南农业学报,2011,24(2). |
| 作者姓名： | 汪茜 胡春锦 黄思良 柯仿钢 |
| 作者单位： | 1. 广西农业科学院微生物研究所,广西南宁,530007 2. 广西农业科学院微生物研究所,广西南宁530007;广西作物遗传改良生物技术重点开放实验室,广西南宁530007 3. 南阳师范学院生命科学与技术学院,河南南阳,473061 4. 佛山盈辉作物科学有限公司,广东佛山,528000 |
| 基金项目： | 广西科研基础平台建设基金项目，广西农科院科技发展基金重点项目 |
| 摘 要： | 采用细菌学方法及分子生物学技术对菌株1505进行鉴定,并对其在防治采后柑橘炭疽病上的效果进行研究.结果显示,菌株1505对柑橘炭疽病菌具有拮抗活性,其活菌悬浮液在PDA平板上对柑橘炭疽病菌产生明显抑制作用,而灭菌发酵液和去除菌体的发酵液均无抑菌效果;连续8次在人工培养基上转代培养,菌株1505对柑橘炭疽病菌生长的抑制力没有发生明显改变;菌株1505对胶孢炭疽菌Colletotrichum gloeosporioides(Penz.)Sacc.]引起的柑橘炭疽病有明显的防治作用,刺伤接种的防效为48.8%,自然发病的防效为46.3%.根据形态学特征和生理生化反应,结合16SrDNA序列分析结果,将菌株1505鉴定为枯草芽孢杆菌(Bacillus subtilis). |
| 关 键 词： | 柑橘炭疽病 胶孢炭疽菌 枯草芽孢杆菌 鉴定 |
Identification of Bio-Control Strain 1505 and Its Effect on Controlling Postharvest Citrus Anthracnose |
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| WANG Qian,HU Chun-jin,HUANG Si-liang,KE Fang-gang.Identification of Bio-Control Strain 1505 and Its Effect on Controlling Postharvest Citrus Anthracnose[J].Southwest China Journal of Agricultural Sciences,2011,24(2). | |
| Authors: | WANG Qian HU Chun-jin HUANG Si-liang KE Fang-gang |
| Institution: | WANG Qian1,HU Chun-jin1,2,HUANG Si-liang3,KE Fang-gang4(1.Microbiology Research Institute,Guangxi Academy of Agricultural Sciences,Guangxi Nanning 530007,China,2.Guangxi Crop Genetic Improvement and Biotechnology Laboratory,3.School of Life Science and Technology,Nanyang Normal University,Henan Nanyang 473061,4.Foshan Yinghui Crop Science Company Limited,Guangdong Foshan 528000,China) |
| Abstract: | The present study aimed to use the bacteriological method and molecular biology technique to identify the strain 1505 and study its efficacy in controlling postharvest Citrus anthracnose.The results showed that the strain 1505 had antagonistic activity against Colletotrichum gloeosporioides.Its cell suspension showed significant suppressive activity against C.gloeosporioides,while no suppressive activity was detected in high-temperature-sterilized fermentation fluid or cell-free fermentation fluid.Less chan... |
| Keywords: | Citrus anthracnose Colletotrichum gloeosporioides Bacillus subtilis Identification |
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