| 家蚕性别相关血液蛋白质组的一维电泳-液相色谱-质谱分析 |
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| 引用本文： | 刘艳艳,池旭娟,阚雪芹,方向明,郑必平,谈建中,平野久.家蚕性别相关血液蛋白质组的一维电泳-液相色谱-质谱分析[J].蚕业科学,2009,35(2). |
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| 作者姓名： | 刘艳艳 池旭娟 阚雪芹 方向明 郑必平 谈建中 平野久 |
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| 作者单位： | 苏州大学建筑与城市环境学院,江苏苏州,215123;苏州大学建筑与城市环境学院,江苏苏州,215123;苏州大学蚕桑研究所,江苏苏州,215123;横滨市立大学,日本横滨,230-0045 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，日本学术振兴会基金 |
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| 摘 要： | 分别从家蚕5龄幼虫和蛹期不同发育阶段提取雌、雄蚕血液总蛋白质,采用一维电泳-液相色谱-质谱(1DE-LC-MS)技术分析家蚕血液蛋白质的差异性表达及差异蛋白组分。在家蚕血液蛋白质含量与种类方面检测到与性别及发育相关的差异性表达,数据库检索结果共获得96个相匹配的候选蛋白质,剔除冗余部分后鉴定其中的27个蛋白质组分,分别属于13种蛋白质或其亚基。雌、雄蚕之间的差异组分主要出现在分子质量70~100 kD之间,其中芳基贮存蛋白、卵黄蛋白原、抗胰凝乳蛋白酶因子为雌特异性表达组分。30K蛋白家族是家蚕血液中高丰度表达的蛋白组分。这些差异蛋白质的鉴定与功能分析为研究家蚕性别发育调控机制提供了新的信息。
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| 关 键 词： | 家蚕 性别 幼虫期 蛹期 血液蛋白质 差异蛋白质组 一维电泳-液相色谱-质谱 |
1DE-LC-MS Analysis of Sex-Related Hemolymph Proteome in Silkworm |
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| LIU Yan-Yan,CHI Xu-Juan,KAN Xue-Qin,FANG Xiang-Ming,Zheng Bi-Ping,TAN Jian-Zhong,HIRANO Hisashi.1DE-LC-MS Analysis of Sex-Related Hemolymph Proteome in Silkworm[J].Acta Sericologica Sinica,2009,35(2). |
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| Authors: | LIU Yan-Yan CHI Xu-Juan KAN Xue-Qin FANG Xiang-Ming Zheng Bi-Ping TAN Jian-Zhong HIRANO Hisashi |
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| Institution: | LIU Yan-Yan1 CHI Xu-Juan1 KAN Xue-Qin1 FANG Xiang-Ming1Zheng Bi-Ping1,2 TAN Jian-Zhong1,2 HIRANO Hisashi3(1School of Architecture , Urban Environment,Soochow University,Suzhou Jiangsu 215123,China,2Institute of Sericulture,3Yokohama City University,Yokohama 230-0045,Japan) |
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| Abstract: | In order to explore the molecular mechanism of sexual development and regulation in silkworm,hemolymph proteins of male and female silkworms were extracted from 5th instar larvae and pupae respectively.After further treatment,these proteins were isolated and identified based on 1DE-LC-MS.The results showed that differential expression of hemolymph proteins on contents and types were detected which is related to sex and developmental stage.96 matchable protein candidates were hit from searches against protei... |
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| Keywords: | Bombyx mori Sex Larval stage Pupal stage Hemolymph protein Differential proteome One-dimensional electrophoresis-liquid chromatography-mass spectrometry(1DE-LC-MS) |
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