Restriction fragment length polymorphism inSolanum spp for the construction of a genetic map ofSolanum tuberosum L.: a preliminary study |
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| Authors: | John J. M. R. Jacobs Frans A. Krens Willem J. Stiekema Martin van Spanje Marinus Wagenvoort |
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| Affiliation: | (1) Centre for Plant Breeding Research (CPO), P.O. Box. 16, 6700 AA Wageningen, the Netherlands |
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| Abstract: | Summary When using two restriction enzymes and DNA probes from different sources, high levels of restriction fragment length polymorphism (RFLP) were observed in Southern blot hybridisation experiments, among 6 di(ha)ploid genotypes ofSolanum tuberosum and some closely related species. In 3 F1 sample populations of 14 individuals each, both heterozygosity and segregation of hybridisation patterns were observed. The non-radioactive biotin-dUTP/SA-AP method for DNA detection on Southern blots proved to be satisfactory and reliable. |
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| Keywords: | biotin potato |
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