The use of a neutralizing monoclonal antibody to detect infections of equine herpesvirus type 2 (EHV-2). |
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| Authors: | A Nordengrahn B Klingeborn A Lindholm M Merza |
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| Affiliation: | SVANOVA Biotech, National Veterinary Institute (SVA), Uppsala Science Park, Sweden. |
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| Abstract: | A blocking enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to equine herpesvirus 2 in serum samples of horses. By measuring the binding to a single epitope, this blocking ELISA gives a good picture of the antibody status in the animal. The test is based on a monoclonal antibody with neutralizing activity and had a sensitivity of 94% and a specificity of 100%. Antibodies due to newly acquired infection in foals were successfully detected with this blocking ELISA. |
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