Potential use of a Yersinia ruckeri O1 auxotrophic aroA mutant as a live attenuated vaccine |
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Authors: | Temprano A Riaño J Yugueros J González P de Castro L Villena A Luengo J M Naharro G |
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Institution: | Departamento de Patología Animal (Sanidad Animal), Microbiología e Inmunología, University of León, Spain. |
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Abstract: | The aroA gene of Yersinia ruckeri, which encodes 5-enolpyruvylshikimate 3-phosphate synthase, was insertionally inactivated with a DNA fragment containing a kanamycin resistance determinant and reintroduced by allelic exchange into the chromosome of Y. ruckeri 21102 O1 by means of the suicide vector pIVET8. The Y. ruckeri aroA::Kan(r) mutant was highly attenuated when inoculated intraperitoneally into rainbow trout, with a 50% lethal dose of >5 x 10(7) CFU. The mutants were not recoverable from the internal organs 48 h post-inoculation or later. The vaccination of rainbow trout with the AroA mutant as a live vaccine conferred significant protection (relative percentage survival = 90%) against the pathogenic wild-type strain of Y. ruckeri. |
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Keywords: | aroA challenge mutant rainbow trout vaccine Yersinia |
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