栒子试管苗的土壤支撑生根培养和带坨移栽

目的]完善栒子试管苗的生根与移栽技术。方法]以粘壤土为培养基支撑物,选用不含大量元素并附加0.75mg/LIBA和15g/L蔗糖的MS液体培养基对栒子试管苗茎段进行生根培养,以琼脂支撑培养为对照,研究不同培养方法栒子试管苗的生根情况及其带坨移栽效果。结果]土壤支撑培养栒子试管苗的生根率（95.0%）、根长（54.3mm）、根数（2.8条）及叶数（7.0片）均显著高于琼脂支撑培养;土壤支撑培养和琼脂支撑培养的栒子试管苗均具有根毛;土壤支撑培养生根栒子试管苗开瓶炼苗21d后带坨移入营养钵中,在移栽后不喷雾、不覆膜、空气相对湿度低至50%的条件下,其成活率高达96.7%。结论]以粘壤土为培养基支撑物可显著提高栒子试管苗的生根及带坨移栽效果。

Soil-supporting Rooting Culture and Lump-transplanting of Cotoneaster hjelmqvistii Test-tube Plantlets

Objective] The purpose was to improve the rooting and transplanting techniques of Cotoneaster hjelmqvistii test-tube plantlets.Method] With clay loam as medium supporter,the liquid MS medium without macro-element and with 0.75 mg/L IBA and 15 g/L sucrose was selected for rooting culture of stem segments of C.hjelmqvistii test-tube plantlets.With agar-supporting culture as CK,the rooting conditions of C.hjelmqvistii test-tube plantlets cultured by different methods and their lump-transplanting effects were studied.Result] The rooting rate,root length,root number and leaf number of C.hjelmqvistii test-tube plantlets in treatment of soil-supporting culture(being 95.0%,54.3 mm,2.8 and 7.0 resp)were significantly higher than those of agar-supporting culture.The C.hjelmqvistii test-tube plantlets in treatment of soil-supporting culture and agar-supporting culture all had root hair.When the rooted C.hjelmqvistii test-tube plantlets in treatment of soil-supporting culture were transferred with lump into nutrient body after open-bottle acclimation for 21 d,their survival rate could reach 96.7% under the condition without spray and film covering and with low relative air humidity of 50%.Conclusion] With clay loam as medium supporter,the rooting and lump-transplanting effects of C.hjelmqvistii test-tube plantlets could be enhanced significantly.