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| 莲雾SRAP反应体系的优化及其应用 | |
| 引用本文： | 蔡元保 杨祥燕 黄明忠 曾黎明 崔明勇 陈显国 林玉虹. 莲雾SRAP反应体系的优化及其应用[J]. 中国农学通报, 2011, 27(22): 214-218 |
| 作者姓名： | 蔡元保 杨祥燕 黄明忠 曾黎明 崔明勇 陈显国 林玉虹 |
| 作者单位： | 1. 广西亚热带作物研究所,南宁,530001 2. 中国热带农业科学院热带作物品种资源研究所,海南儋州,571737 |
| 基金项目： | 广西壮族自治区直属公益性科研院所基本科研业务费专项资金项目(桂热研201102) |
| 摘 要： | 以莲雾DNA为模板，应用正交设计法对SRAP反应体系中的各个主要影响因素进行了优化筛选。结果表明，20 μL反应体系中各组分的最适浓度或用量分别为：1×Buffer，3.0 mmol/L Mg2+，0.3 mmol/L dNTPs，0.4 μmol/L引物，0.5 U Taq DNA聚合酶和40 ng模板DNA。利用该优化体系通过64对SRAP引物组合对7份连雾进行了SRAP-PCR扩增，证实了该体系具有稳定可靠、重复性好、多态性较强等特点。 |
| 关 键 词： | 木材 木材 阻燃剂 增重率 失重率 燃烧性 浸渍处理 |
| 收稿时间： | 2011-04-25 |
| 修稿时间： | 2011-05-19 |
Optimization of SRAP-PCR System and its Application in Wax Apple (Syzygium samarangens) |
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| Cai Yuanbao,Yang Xiangyan,Huang Mingzhong,Zeng Liming,Cui Mingyong,Chen Xianguo,Lin Yuhong. Optimization of SRAP-PCR System and its Application in Wax Apple (Syzygium samarangens)[J]. Chinese Agricultural Science Bulletin, 2011, 27(22): 214-218 | |
| Authors: | Cai Yuanbao Yang Xiangyan Huang Mingzhong Zeng Liming Cui Mingyong Chen Xianguo Lin Yuhong |
| Affiliation: | Cai Yuanbao 1,Yang Xiangyan 1,Huang Mingzhong 2,Zeng Liming 1,Cui Mingyong 1,Chen Xianguo 1,Lin Yuhong 1(1 Guangxi Sub-tropical Crops Research Institute,Nanning 530001,2 Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou Hainan 571737) |
| Abstract: | The main influential factors of SRAP reaction system were optimized for genomic DNA in wax apple（Syzygium samarangens） by orthogonal design.The results showed that the optimum concentration was 1× Buffer,Mg 2＋ 3.0 mmol/L,dNTPs 0.3 mmol/L,primer 0.4 μmol/L,Taq DNA polymerase 0.5 U,40 ng template DNA with a total volume of 20 μL reaction solution.The molecular identification of 7 varieties of wax apple were achieved by 64 SRAP primer combinations with this optimum system which could provide clear,reliable and repeatable abundant polymorphisms molecular markers. |
| Keywords: | wax apple(Syzygium samarangens) SRAP orthogonal design optimization |
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