| 猪骨和皮脂性状相关ESTs的SNPs筛查与关联分析 |
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| 引用本文： | 覃立立,蔡更元,张豪,陈瑶生,李加琪,王翀.猪骨和皮脂性状相关ESTs的SNPs筛查与关联分析[J].中国农业科学,2011,44(16):3403-3412. |
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| 作者姓名： | 覃立立 蔡更元 张豪 陈瑶生 李加琪 王翀 |
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| 作者单位： | 1. 华南农业大学动物科学学院/广东省农业动物基因组学与分子育种重点实验室;
2. 广东省农业科学院畜牧研究所;
3. 中山大学有害生物控制与资源利用国家重点实验室 |
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| 基金项目： | 广东省自然科学基金资助项目(9251064201000005); 广东省科技攻关项目(2008B020600009); 广东省生猪产业技术体系建设专项 |
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| 摘 要： | 【目的】性状的QTL位于多条染色体上，寻找猪骨和皮脂性状的新候选基因，为育种工作提供参考。【方法】本研究从NCBI搜索到可能与骨和皮脂性状相关的ESTs 177条，通过cDNA池法测序筛选到其中9条ESTs上有21个SNPs，在长－蓝家系F2代进行关联分析，并用辐射杂交细胞系技术对其中的4条ESTs进行定位。【结果】发现其中6个SNPs与猪的骨和皮脂性状相关。E53不同基因型在后躯骨重上的差异达到了极显著水平（P＜0.01），在骨率、前躯骨重和皮脂率上的差异均达到了显著水平（P＜0.05）。E82不同基因型在中躯骨重、中躯皮脂重上的差异均达到了极显著水平（P＜0.01），在骨率、皮脂率、前躯皮脂重上的差异达到显著水平（P＜0.05）。E36在前躯骨重、后躯皮脂重，E42在中躯皮脂重、后躯皮脂重，E54在中躯骨重，E87在前躯骨重、皮脂率上不同基因型差异均达到了显著水平（P＜0.05）。E36定位在猪10q13处；E53定位在4q21-23处；E54定位在10号染色体SWC19处；E87定位在9p21处。【结论】E53和E82所在的基因有可能作为影响骨和皮脂性状的新候选基因进行而深入研究。
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| 关 键 词： | ESTs SNPs 骨性状 皮脂性状 |
| 收稿时间： | 2010-07-26 |
SNPs Screening and Correlation Analysis of ESTs Associated With Bone and Sebum Traits of Pigs |
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| QIN Li-li,CAI Geng-yuan,ZHANG Hao,CHEN Yao-sheng,LI Jia-qi,WANG Chong.SNPs Screening and Correlation Analysis of ESTs Associated With Bone and Sebum Traits of Pigs[J].Scientia Agricultura Sinica,2011,44(16):3403-3412. |
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| Authors: | QIN Li-li CAI Geng-yuan ZHANG Hao CHEN Yao-sheng LI Jia-qi WANG Chong |
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| Institution: | QIN Li-li1,CAI Geng-yuan2,ZHANG Hao1,CHEN Yao-sheng3,LI Jia-qi1,WANG Chong1(1College of Animal Science,South China Agricultural University/Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding,Guangzhou 510642,2Institute of Animal Science,Guangdong Academy of Agricultural Science,Guangzhou 510640,3Sun Yat-Sen University,State Key Laboratory of Biocontrol,Guangzhou 510006) |
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| Abstract: | 【Objective】 QTLs are located in several chromosomes. In order to provide a reference, new candidate genes of bone and sebum traits of pigs were screened.【Method】One hundred and seventy-seven ESTs that may be associated with bone and sebum traits have been searched from NCBI and 21 SNPs were screened by cDNA pool sequencing in 9 ESTs. Four ESTs were located by RH (radiation hybrid). 【Result】 It was discovered that 6 SNPs that are related to bone and sebum traits actually exist in the F2 crossbred population of the Landrace pigs and Lantang pigs after the correlation analysis. E53 is extremely significantly correlated with rear bone (ReB) (P＜0.01)and significantly correlated with bone propotion (BP), fore bone (ForeB) and sebum fat propotion (SFP) (P＜0.05).E82 is extremely significantly correlated with mid-bone (MidB), mid-sebum fat (MidSF) (P＜0.01) and significantly correlated with bone propotion (BP), sebum fat propotion (SFP) and fore sebum fat (ForeSF) (P＜0.05). The differences of E36 in rear sebum fat (ReSF) and fore bone (ForeB), E42 in mid-sebum fat (MidSF) and rear sebum fat (ReSF), E54 in mid-bone (MidB), E87 in fore bone (ForeB) and sebum fat propotion (SFP) reached the remarkable level (P＜0.05). E36 is located on 10q13. E53 is located on 4q21-23. E54 is located on SWC19 of the chromosome 10 without the specific position. E87 is located on 9p21.【Conclusion】E53 and E82 need to be studied further as new candidate genes which affect the bone and sebum traits of pigs. |
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| Keywords: | ESTs SNPs bone traits sebum traits |
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