A Standard Reference Diet for Crustacean Nutrition Research. II. Selection of a Purification Procedure for Production of the Rock Crab Cancer irroratus Protein Ingredient |
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| Authors: | John D Castell J C Kean D G C McCann Andrew D Boghen Douglas E Conklin Louis R D'Abramo |
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| Institution: | Department of Fisheries and Oceans, Fisheries and Environmental Sciences, Halifax Fisheries Research Laboratory, P.O. Box 550, Halifax, Nova Scotia B3J 2S7, Canada;Biology Department, Dalhousie University, Halifax Nova Scotia B3H 4H6. Canada;Department de Biologie, Universite de Moncton, Moncton, New Brunswick E1A 3E9, Canada;University of California, Davis Campus, Bodega Bay Marine Laboratory, P.O. Box 247, Bodega Bay, California 94923 USA;Department of Wildlife and Fisheries, Mississippi State University, P.O. Drawer LW, Mississippi State, Mississippi 39762 USA |
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| Abstract: | Obtaining reliable results from comparative crustacean nutrition studies requires a dietary reference protein which is available world-wide and is nutritionally optimal for growth and survival. Traditional protein sources include "vitamin free" casein, egg albumin, whole egg protein, soybean protein, and others. These have proven to be inadequate for juvenile lobster ( Homrus americanus ). Purified rock crab ( Cancer irroratus ) protein is proposed to be nutritionally superior to casein for inclusion in semi-purified lobster diets.
Eighteen week feeding trials with juvenile H. americanus were conducted to compare diets containing protein obtained from rock crabs ( Cancer irroratus ), and produced by several different purification processes with a control diet having casein as its primary protein source. Diets containing purified rock crab protein were found to be superior to the control diet. Among the differently derived purified rock crab protein products, the precooked, isopropanol-purified was the most effective dietary ingredient. |
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