| LH抗体间接ELISA检测方法的建立 |
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| 引用本文： | 崔贞亮,李红飞,吴庆侠,靳亚平,刘璇. LH抗体间接ELISA检测方法的建立[J]. 西北农业学报, 2007, 16(3): 26-28 |
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| 作者姓名： | 崔贞亮 李红飞 吴庆侠 靳亚平 刘璇 |
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| 作者单位： | 1. 西北农林科技大学动物科技学院,农业部家畜内分泌与胚胎工程重点开放实验室,陕西杨凌,712100
2. 河南省商丘市梁园区动物检疫站,河南商丘,476000 |
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| 基金项目： | 西北农林科技大学校科研和教改项目 |
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| 摘 要： | 以高纯度的促黄体素（LH）作为包被抗原,建立了检测LH抗体的间接ELISA法,该法的最适抗原包被浓度为1μg/mL,最佳酶标山羊抗小鼠抗体稀释度为1∶1500.该法只与LH阳性小鼠血清呈现阳性反应,而与LH阴性小鼠血清和小鼠促卵泡素（FSH）阳性血清呈现阴性反应.结果表明,该法具有良好的特异性和重复性,可用于LH免疫后抗体水平检测.
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| 关 键 词： | 间接ELISA LH抗体 小鼠 |
| 文章编号： | 1004-1389（2007）03-0026-03 |
| 收稿时间： | 2006-06-07 |
| 修稿时间： | 2006-06-072006-11-23 |
Development of Indirect ELISA for Detecting LH Antibody |
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| CUI Zhen-liang,LI Hong-fei,WU Qing-xi,JIN Ya-ping and LIU Xuan. Development of Indirect ELISA for Detecting LH Antibody[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2007, 16(3): 26-28 |
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| Authors: | CUI Zhen-liang LI Hong-fei WU Qing-xi JIN Ya-ping LIU Xuan |
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| Affiliation: | Key Open Laboratory of Animal Reproductive Endocrinology and Embryo Engineering of the Ministry of Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;Key Open Laboratory of Animal Reproductive Endocrinology and Embryo Engineering of the Ministry of Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;Key Open Laboratory of Animal Reproductive Endocrinology and Embryo Engineering of the Ministry of Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;Key Open Laboratory of Animal Reproductive Endocrinology and Embryo Engineering of the Ministry of Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;Animal Quarantine Station of Liangyuan area, Shangqiu Henan 476000, China |
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| Abstract: | |
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| Keywords: | Indirect ELISA LH antibody Mouse |
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