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| 单层细胞共培养对猪去卵丘卵母细胞体外成熟和孤雌发育的影响 | |
| 引用本文： | 顾晓龙,孟祥黔,吴彩凤,戴建军,张廷宇,谢一妮,吴志强,刘亮,曾宪垠,张德福.单层细胞共培养对猪去卵丘卵母细胞体外成熟和孤雌发育的影响[J].中国兽医学报,2011,31(4). |
| 作者姓名： | 顾晓龙 孟祥黔 吴彩凤 戴建军 张廷宇 谢一妮 吴志强 刘亮 曾宪垠 张德福 |
| 作者单位： | 1. 四川农业大学生命科学与理学院,四川雅安625014;上海农业科学院畜牧兽医研究所,上海201106;上海农业遗传育种重点实验室动物遗传工程研究室,上海201106 2. 上海农业科学院畜牧兽医研究所,上海201106;上海农业遗传育种重点实验室动物遗传工程研究室,上海201106 3. 四川农业大学生命科学与理学院,四川雅安,625014 |
| 基金项目： | 上海市科技兴农推广项目(2007-3-7);上海市科技兴农重点攻关项目(2005-3-5); 转基因生物新品种培育重大专项(2009ZX08006-014B;2008ZX08006-005); 上海市农科院青年科技发展基金(2008(07)); 上海市科委科技成果转化项目(103919N1800); 上海市农业遗传育种重点实验室开方课题(Shagb2008-03) |
| 摘 要： | 本试验比较观察第一极体(The first polar body,PbⅠ)、Oosight imaging system观察和hocchst33342染色法对第2次减数分裂中期(Metaphase Ⅱ,MⅡ)卵母细胞判定结果的相关性分析,并探讨卵巢皮质细胞(porcine ovarian cortex cells,pOCCs)、猪输卵管上皮细胞(porcine oviductal epithelial cells,pOECs)和猪卵丘颗粒细胞(porcine cumulus cells,pCCs)等3种单层细胞体外共培养体系对猪去卵丘卵母细胞(cumulus cells denuded oocytes,Dos)体外成熟(in vitro maturation,IVM)和孤雌发育的影响。结果显示:(1)Oosight imaging system判定卵母细胞成熟的结果与hocchst33342染色法判定的结果有很强相关性(R=0.973,P<0.01,N=90);(2)pOCCs单层细胞共培养体系中猪去卵丘卵母细胞成熟率显著高于pOECs((52.5±0.30)%vs(43.8±2.18)%,P<0.05),且... |
| 关 键 词： | 纺锤体观测系统 共培养 去卵丘卵母细胞 体外成熟 |
Effects of cell monolayer co-culture on porcine cumulus cells denuded oocytes during in vitro maturation and parthenogenetic development |
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| GU Xiao-long,MENG Xiang-qian,WU Cai-feng,DAI Jian-jun,ZHANG Ting-yu,XIE Yi-ni,WU Zhi-qang,LIU Liang,ZENG Xian-yin,ZHANG De-fu.Effects of cell monolayer co-culture on porcine cumulus cells denuded oocytes during in vitro maturation and parthenogenetic development[J].Chinese Journal of Veterinary Science,2011,31(4). | |
| Authors: | GU Xiao-long MENG Xiang-qian WU Cai-feng DAI Jian-jun ZHANG Ting-yu XIE Yi-ni WU Zhi-qang LIU Liang ZENG Xian-yin ZHANG De-fu |
| Institution: | GU Xiao-long1,2,3,MENG Xiang-qian1,3#,WU Cai-feng2,DAI Jian-jun2,ZHANG Ting-yu2,XIE Yi-ni1,WU Zhi-qang1,LIU Liang2,ZENG Xian-yin1*,ZHANG De-fu2,3*(1.Animal Science & Technology College,Sichuan Agricultural University,Ya'an,Sichuan 625014,China,2.Institute of Animal Science and Veterinary Medicine,Shanghai Academy of Agricultural Science,Shanghai 201106,3.Division of Animal Genetic Engineering,Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding,China) |
| Abstract: | The objective of the experiment was to compare the correlation of three ways to determine matured MII oocytes,including the first polar body,oosight imaging system observation and hocchst33342 staining,and to evaluate the effects of pOCCs,pOECs and pCCs monolayer co-culture system on porcine cumulus cells denuded oocytes during in vitro maturation and parthenogenesis,respectively.The results show that:(1)determining the outcome of oocyte maturation,the result of oosight imaging system is significant correla... |
| Keywords: | oosight imaging system co-culture cumulus cells denuded oocytes in vitro mature |
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