| 3种兔球虫18S rDNA部分序列测定与系统发育分析 |
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| 引用本文： | 方素芳,顾小龙,崔平.3种兔球虫18S rDNA部分序列测定与系统发育分析[J].中国兽医学报,2011,31(6). |
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| 作者姓名： | 方素芳 顾小龙 崔平 |
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| 作者单位： | 河北北方学院,动物科学院,河北张家口075000 |
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| 基金项目： | 国家兔产业技术体系(农业部)资助项目 |
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| 摘 要： | 采用单卵囊分离法从河北某兔场分离大型艾美耳球虫、黄艾美耳球虫及肠艾美耳球虫,接种无球虫兔后获得大量纯种卵囊,CTAB法提取孢子化卵囊基因组DNA。利用艾美耳属球虫18S rDNA保守引物,PCR扩增3种兔球虫18S rDNA片段,产物纯化后测序。将3种球虫18S rDNA测序结果与GenBank中发布的兔球虫18S rDNA序列用DNAStar软件进行比对。使用MEGA4.0软件对兔球虫18S rDNA进行同源性比较,并绘制遗传进化树。结果表明,大型艾美耳球虫扩增出大小为1 521bp的18S rDNA片段;黄艾美耳球虫及肠艾美耳球虫均扩增出大小为1 520bp的18S rDNA片段。序列比对结果显示,3种河北株兔球虫与GenBank中相应的3种兔球虫18S rD-NA(EF694016、EF694011、EF694012)相似性分别为99.6%、99.6%和100%。3种河北株兔球虫序列和GenBank中兔球虫18S rDNA序列(EF694007-EF694017)位于一个单系集群。
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| 关 键 词： | 大型艾美耳球虫 黄艾美耳球虫 肠艾美耳球虫 18S rDNA 测序 遗传进化树 |
The sequence and phylogenetic analysis of 18S rDNA from three species of rabbit coccidia |
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| FANG Su-fang,GU Xiao-long,CUI Ping.The sequence and phylogenetic analysis of 18S rDNA from three species of rabbit coccidia[J].Chinese Journal of Veterinary Science,2011,31(6). |
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| Authors: | FANG Su-fang GU Xiao-long CUI Ping |
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| Institution: | FANG Su-fang,GU Xiao-long,CUI Ping(Colloge of Animal Science and Technology,Hebei North University,Zhangjiakou,Hebei 075000,China) |
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| Abstract: | To further determine E.magna,E.flavescens and E.intestinalis,three species of rabbit coccidia were isolated from rabbits in Hebei and purified.Genomic DNA were extracted from their sporulated oocysts by the method of CATB.Using conservative primer of 18S rDNA of Eimeria,18S rDNA gene fragment of three spccies of rabbit coccidia were amplified and sequenced,then they was analysed by DNAStar and aligned with corresponding sequence of the other eleven species of rabbit-infecting Eimeria in the GenBank,the phyl... |
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| Keywords: | E magna E flavescens E intestinalis 18S rDNA sequence phylogenetic tree |
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