三种喹诺酮类药物在鸡蛋中残留消除规律的研究

建立了一种检测三种喹诺酮类药物在鸡蛋中残留的高效液相色谱-荧光检测法。利用建立的方法对三种喹诺酮类药物在产蛋期蛋鸡用药后鸡蛋中的浓度进行了测定，并根据测定值研究了鸡蛋中药物的残留消除规律。400kg水中分别添加不同含量的诺氟沙星、氧氟沙星、恩诺沙星．连续给药3d，使用高效液相色谱法对鸡蛋中三种喹诺酮类药物残留进行分析。结果表明，停药8d时，鸡蛋中检出的诺氟沙星和氧氟沙星的浓度均低于10μg/kg；停药12d时，鸡蛋中检出的恩诺沙星的浓度低于10μg／蚝。建议诺氟沙星和氧氟沙星在临床上蛋鸡产蛋前10d左右停用，恩诺沙星在临床上蛋鸡产蛋前14d左右停用。

Studies on elimination rule on residues of three kinds of quinolones in eggsZhang min,, Ning Jun,Yu Junjun, Wei Tian,Tai Di

A method for determination of quinolones residues in eggs by HPLC with fluorescence detector detection was established, and this method for determination the concentration in the laying hens, the elimination of quinolones residues in eggs was studied. Different content of norfloxacin, ofloxacin, enrofloxacin were added in the water for 3 days, the residues of quinolones were determined by HPLC. The results showed that the residues of norfloxacinand and ofloxacin in eggs were less than 10μg/kg at 8th withdrawal day;the residues of enrofloxacin in eggs was less than 10μg/kg at 12th withdrawal day. So, the withdrawal period of norfloxacin, ofloxacin, enrofloxacin were found, to be 10 d, 10 d, 14 d in clinical before the age at first egg, respectively.