Prolamin Analysis of Progenies from Androgenetic Plants of Triticale

The present work reports results of the analysis of variability of prolamins in five breeding lines of triticale and 61 androgenetic progenies obtained from them by in vitro culture of the anthers. The phenotypic analysis of the prolamins showed heterogeneity even between seeds harvested from the same plant regenerated by in vitro culture. The variability of prolamins in this material could be, perhaps, explained under different hypotheses: unreduced microspores, embryo fusion. The percentage of androgenetic plants which generated uniform progenies for the prolamins was 14.7. The plants obtained by in vitro culture showed a significant increase of the phenotypic variability for both specific bands and patterns of prolamins as compared with the parental materials. Some plants showed missing groups of prolamins in their electrophoretic profiles, which completely affected specific regions of the proteinogram. This could be explained by gametoclonal variation based on either structural rearrangements, or aneuploidy affecting chromosomes carrying genes involved in the expression of prolamins.