| 哺乳动物细胞无血清培养基研究进展 |
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| 引用本文： | 杨学义,刘飞,向双云,周珍辉.哺乳动物细胞无血清培养基研究进展[J].动物医学进展,2011,32(2):69-72. |
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| 作者姓名： | 杨学义 刘飞 向双云 周珍辉 |
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| 作者单位： | 洛阳师范学院生命科学系;北京农业职业学院畜牧兽医系; |
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| 基金项目： | 洛阳师范学院省部级以上培育基金项目(2008-PYJJ-014);洛阳师范学院应用科学与技术研究基金项目(2009-YYJS-02); 河南省科技攻关项目(102102110156) |
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| 摘 要： | 随着哺乳动物细胞培养种类、规模和应用范围的不断扩大以及安全性能要求的不断提高,无血清培养基的研制已经成为细胞工程领域的重要课题.许多类型的哺乳动物细胞无血清培养基配方得到了研究开发和优化,且有些已经进入第3代无蛋白无血清培养基研制阶段.论文重点概述了无血清培养基在添加因子作用机制、细胞分化条件、细胞和组织移植以及肿瘤治...
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| 关 键 词： | 无血清培养基 细胞工程 哺乳动物 |
Progress on Serum-free Medium for Mammalian Cells |
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| YANG Xue-yi,LIU Fei,XIANG Shuang-yun,ZHOU Zhen-hui.Progress on Serum-free Medium for Mammalian Cells[J].Progress In Veterinary Medicine,2011,32(2):69-72. |
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| Authors: | YANG Xue-yi LIU Fei XIANG Shuang-yun ZHOU Zhen-hui |
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| Institution: | YANG Xue-yi1,LIU Fei1,XIANG Shuang-yun2,ZHOU Zhen-hui2(1.Agriculture Biotechnology Research Centre,Life Science Department of Luoyang Normal College,Luoyang,Henan,471022,China,2.Department of Animal Husbandry and Veterinary Medicine,Beijing Agricultural Vocation College,Beijing,102442,China) |
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| Abstract: | With the expanding species,scale and applications of mammalian cell culture,the development of serum-free media have become a major task in the field of cell engineering.Many types of serum-free media for mammalian cells have been established and optimized,and there has emerged the 3-generation serum-free meda named protein-free media.The present study focused on the latest research progress on serum-free media used in the studies on the mechanisms of the added factors,cell differentiation conditions,cell/t... |
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| Keywords: | serum-free medium cell engineering mammal |
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