竹荚鱼属鱼类线粒体DNA控制区结构及其系统发育分析

采用PCR产物直接测序法测定了2尾来自中国南海的日本竹煲鱼（Trachurus japonicus）线粒体DNA控制区基因全序列，并结合从GenBank中下载的8种竹笑鱼属相应序列，对竹煲鱼属控制区序列进行了结构分析，识别出终止序列区、中央区和保守序列区3个区域，并找到了2个与DNA复制终止相关的序列TAS和中央保守区的保守序列CSB—F、CSB—E和CSB—D，以及保守序列区的保守序列CSBl、CSB2和CSB3。以鳜（Sinipercachuatsi）作为外群，使用邻接法和最大简约法构建了9种竹荚鱼属的系统发育树。竹笑鱼属鱼类为单系类群，蓝竹煲鱼（rpicturatus）、智利竹笑鱼（zmurphyi）、太平洋竹笑鱼（zsymmetricus）、南非竹荚鱼（rcapensis）和竹荚鱼（rtrachurus）构成一支，地中海竹笑鱼（zmediterraneus）、青背竹笑鱼（zdeclivis）、日本竹荚鱼（rjaponicus）和新西兰竹荚鱼（rnovaezelandiae）为另一支；日本竹笑鱼未单独成一支，而是聚人青背竹笑鱼和新西兰竹笑鱼之间，初步猜测日本竹笑鱼和新西兰竹荚鱼为同种异名。

Structure and phylogenetic analysis of mitochondrial DNA control region of genus Trachurus

We used PCR to amplify the complete sequences of mitochondrial DNA （mtDNA） control region of 2 Trachurus japonicus from South China Sea, and then aligned them by Clustal X with 8 other species of the genus Trachurus from GenBank. According to the alignment and structure comparison, the mtDNA control region of the genus Trachurus can be divided into 3 domains ： termination asso- ciated sequence domain （TAS） , central conserved domain （CD） and conserved sequence block domain （CSB）. We identified 2 ter- mination-associated sequences （TAS） and 3 conserved blocks （CSB-F, CSB-E and CSB-D） in CD domain as well as 3 conserved se- quence blocks （ CSB-1, CSB-2 and CSB-3 ） in CSB domain. Taking Siniperca chuatsi as outgroup, the molecular phylogenetic relation- ship among 9 Carangidae was analyzed using neighbor-joining （NJ） method and maximum parsimony method in MEGA 4. 0. Results show that the genus Trachurus fishes belong to a monophyletic group： T. picturatus, T. murphyi, T. symmetricus, T. capensis and T. trachurus gather into one cluster; T. mediterraneus, T. declivis, T. japonicus and T. novaezelandiae gather another. T. japonicus does not form into an independent group but is merged into T. declivis and T. novaezelandiae. Thus, it is suggested that T. japonicus and T. novaezelandiae may be 2 synonyms.