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Detection of ribosomal RNA genes by chemiluminescence inSolanum tuberosum L.: a rapid and non-radioactive technique for the characterisation of potato germplasm
Authors:K Harding
Institution:(1) Department of Genetics, School of Biological Sciences, Medical School, University of Nottingham, NG7 2UH Nottingham, UK;(2) Cell and Molecular Genetics Department, c/o Dr Kumar, Scottish Crop Research Institute, Invergowie, DD2 5DA Dundee, Scotland, UK
Abstract:Summary The characterisation of potato germplasm is important for the exploitation of this crop, and DNA techniques are playing an increasingly important role as valuable tools in the evaluation of agronomic characters. The rapid, non-radioactive detection of the ribosomal RNA gene fragments which identify restriction fragment length polymorphisms (RFLPs) in two cultivars ofSolanum tuberosum L. is described.
Keywords:AMPPD  avidin  biotin  dioxetane  rDNA  RFLPs
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