| 牦牛皮蝇幼虫的RFLP快速鉴定 |
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| 引用本文： | 刘浩浩,李玉萍,黄志宏,徐亚欧,金素钰,林亚秋,郑玉才.牦牛皮蝇幼虫的RFLP快速鉴定[J].湖北农业科学,2012,51(7):1477-1480. |
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| 作者姓名： | 刘浩浩 李玉萍 黄志宏 徐亚欧 金素钰 林亚秋 郑玉才 |
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| 作者单位： | 1. 西南民族大学生命科学与技术学院,成都,610041
2. 四川省九龙县农牧局,四川九龙,622000 |
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| 摘 要： | 从牦牛皮下采集皮蝇(Hypoderma)幼虫,用DNA提取试剂盒、Chelex-100两种方法提取幼虫的基因组DNA；通过PCR扩增线粒体细胞色素氧化酶Ⅰ基因(CO Ⅰ)中的高度可变序列,扩增产物经BfaⅠ酶切后用琼脂糖凝胶检测,结果表明所采集的皮蝇幼虫属于同一种.测序结果显示,扩增的序列与GenBank 中中华皮蝇(H.sinense)相应序列的相似性为99.56％,表明所采集的皮蝇幼虫均为中华皮蝇.
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| 关 键 词： | 牦牛 皮蝇(Hypoderma)幼虫 RFLP 细胞色素氧化酶Ⅰ 鉴定 |
RFLP Rapid Identification of Hypoderma Larva Affecting Yaks |
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| LIU Hao-hao
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LI Yu-ping
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HUANG Zhi-hong
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XU Ya-ou
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JIN Su-yu
,
LIN Ya-qiu
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ZHENG Yu-cai.RFLP Rapid Identification of Hypoderma Larva Affecting Yaks[J].Hubei Agricultural Sciences,2012,51(7):1477-1480. |
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| Authors: | LIU Hao-hao LI Yu-ping HUANG Zhi-hong XU Ya-ou JIN Su-yu LIN Ya-qiu ZHENG Yu-cai |
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| Institution: | 1(1.College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China; 2.Animal Husbandry Bureau of Jiulong County,Jiulong 622000,Sichuan,China) |
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| Abstract: | RFLP and sequencing was used to identify the Hypoderma larvae collected from yaks.DNA of larvae was extracted by Genomic DNA Extraction Kit and Chelex-100 method.The variable region of the mitochondrial cytochrome oxidase Ⅰ gene(COⅠ) was amplified by PCR.BfaⅠ digestion and agarose gel electrophoresis revealed that the collected larvae belonged to one species.Sequencing and alignment showed that the amplified sequence shared 99.56% homology with that of H.sinense in GenBank,suggesting that the collected larvae were H.sinense. |
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| Keywords: | yak Hypoderma larva RFLP cytochrome oxidase Ⅰ identification |
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