杂交中稻广两优476品种与纯度的鉴定

为辨别杂交中稻广两优476种子真伪并鉴定其纯度,利用农业部《水稻品种鉴定DNA指纹方法》(NY/T 1433-2007)中推荐的24对引物进行PCR,使用聚丙烯酰胺凝胶电泳对广两优476及其亲本,以及另外几个育种常用素本材料进行了多态性筛选,并利用筛选所得引物的SSR标记对广两优476进行了种子纯度分析.结果表明,这24对引物在几个常用亲本及广两优476间具有良好的多态性,可用于构建广两优476的DNA指纹图谱.同时,从中筛选得到标记RM209和RM17的两对引物在广两优476父母本间有显著多态性,其中RM209的引物的PCR产物在2％琼脂糖凝胶电泳条件下分辨效果最好,可用于广两优476杂交种子的纯度鉴定.2009年冬在实验室使用RM209鉴定广两优476杂交种子纯度,结果为98.6％,与2010年春在海南田间种植鉴定纯度98.3％相符.

The Variety and Purity Indentification of Hybrid Rice Guangliangyou 476

24 SSR markers recommended by China National Rice Research Institute were screened;and the markers showing polymorphism among hybrid rice parents of Guangliangyou 476 and other popular cultivars were used for purity test of Guangliangyou 476 seeds.It was showed that the SSR markers were with great polymorphism among the parents and Guangliangyou 476,thus were suitable for building of fingerprint of Guangliangyou 476.2 SSR markers,RM209 and RM17,were detected with significant polymorphism between R476 and Guangzhan63S;moreover,bands of RM209 were well departed by 2% agarose gel electrophoresis,making it suitable for the purity test of Guangliangyou 476 seeds.The laboratory tested purity of Guangliangyou 476 hybrid seed in winter 2009 using RM209 was 98.6%,which was consistent with the filed tested result in Hainan spring 2010(98.3%).