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| 中华圆田螺纤维素酶提取及纯化工艺的优化 | |
| 引用本文： | 马力通,李珺,刘佼.中华圆田螺纤维素酶提取及纯化工艺的优化[J].湖北农业科学,2012,51(13):2812-2814. |
| 作者姓名： | 马力通 李珺 刘佼 |
| 作者单位： | 1. 内蒙古自治医生物质能源化利用重点实验室,内蒙古包头014010;内蒙古科技大学生物工程技术研究所,内蒙古包头014010 2. 内蒙古自治医生物质能源化利用重点实验室,内蒙古包头,014010 3. 内蒙古科技大学生物工程技术研究所,内蒙古包头,014010 |
| 基金项目： | 内蒙古科技大学创新基金 |
| 摘 要： | 以中华圆田螺(Cipangopaludina cahayensis)为原料提取纤维素酶,采用均匀设计优化提取工艺,并采用硫酸铵沉淀法初步纯化酶液.结果表明,中华圆田螺纤维素酶提取最佳工艺为缓冲液pH 4.8、浸提时间12 min、离心时间24 min；采用饱和度45％的硫酸铵沉淀法纯化酶液,所得酶液的总酶活和比活力最高,分别为651.01 U和34.099 U/g. |
| 关 键 词： | 中华圆田螺(Cipangopaludina cahayensis) 纤维素酶 提取 均匀设计 硫酸铵沉淀 |
Optimization of Extraction and Purification Technology of Cellulase from Cipangopaludina cahayensis |
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| MA Li-tong , LI Jun , LIU Jia.Optimization of Extraction and Purification Technology of Cellulase from Cipangopaludina cahayensis[J].Hubei Agricultural Sciences,2012,51(13):2812-2814. | |
| Authors: | MA Li-tong LI Jun LIU Jia |
| Institution: | 1.Inner Mongolia Key Laboratory of Biomass-Energy Conversion,Baotou 014010,Inner Mongolia,China;2.Institute of Bioengineering and Technolgy,Inner Mongolia University of Science and Technology,Baotou 014010,Inner Mongolia,China) |
| Abstract: | Cellulase was extracted from Cipangopaludina cahayensis.The extraction conditions was optimized by uniform design;and the cellulose extracted was purified by ammonium sulfate precipitation.The results showed that the optimum conditions for cellulase extraction were buffer pH 4.8;extraction time,12 min;centrifugal time,24 min.And the best saturation of ammonium sulfate for enzyme purification was 45%,as the enzymatic activity and specific activity was 651.01 U and 34.099 U / g,respectively. |
| Keywords: | Cipangopaludina cahayensis cellulase extraction uniform design ammonium sulfate precipitation |
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