牛磺脱氧胆酸钠和CpGDNA对灭活H9N2禽流感病毒鼻腔免疫鸭的呼吸道抗体分泌细胞的影响

运用牛磺脱氧胆酸钠和CpG DNA配合鸭源禽流感H9N2灭活病毒滴鼻免疫雏鸭,通过检测鸭呼吸道各组织中IgA和IgG抗体分泌细胞面积的变化对免疫效果进行评价.结果发现:应用牛磺脱氧胆酸钠和CpG DNA配合H9N2灭活病毒鼻腔免疫后3、5和7周,鼻腔、气管和气管叉组织中的IgA和IgG分泌细胞面积显著或极显著(P＜0.05或P＜0.01)高于单独H9N2灭活病毒免疫后的水平；应用CpG DNA配合H9N2灭活病毒鼻腔免疫后鼻腔和气管组织中IgA和IgG分泌细胞的面积有部分提高(P＜0.05)；而单独运用H9N2灭活病毒鼻腔免疫后IgA和IgG分泌细胞面积同对照组相比没有显著差异.结果表明:在牛磺脱氧胆酸钠和CpG DNA的配合下,禽流感H9N2灭活病毒鼻腔免疫能够提高雏鸭呼吸道组织中IgA分泌细胞和IgG分泌细胞的面积,有效地增强呼吸道局部的免疫应答水平.

Co-administration with Inactivated Avian Influenza Virus and Adjuvants Enhances the Antibody Secreting Cells in Duck Respiratory Tract

Ducks were intranasal immunized with inactivated avian influenza H9N2 virus(IAIV) in combination with sodium taurodeoxycholate hydrate and CpG DNA.Distribution and number of antibody secreting cells in duck respiratory tract were studied.Results showed that 3,5 and 7 weeks after vaccination with sodium taurodeoxycholate hydrate and CpG DNA,the area of IgA and IgG secreting cells of nasal cavity,trachea and tracheal bifurcation were increased significantly(P<0.05 or P<0.01) compared with that of ducks with IAIV alone.After intranasal immunization with IAIV and CpG DNA,the area of IgA and IgG secreting cells of nasal cavity and trachea were partly increased(P<0.05).While no significant change on the area of IgA and IgG antibody secreting cells of respiratory tract tissues was observed in respiratory tract between ducks with IAIV alone and PBS.It demonstrated that intranasal immunization with IAIV combined with sodium taurodeoxycholate hydrate and CpG DNA could increase the area of IgA antibody secreting cells and IgG antibody secreting cells and enhance the level of local humoral immune response of duck’s respiratory tract.