乳酸锌对猪空肠上皮细胞IPEC-J2增殖及相关调控基因mRNA表达的影响

旨在探讨乳酸锌对猪空肠上皮细胞增殖及相关调控基因ZnT2、DMT1、IREG-1、MT1和ZIP4 mRNA表达的影响.用乳酸锌的锌浓度分别为50、100、150、200 mg·L-1的培养基培养IPEC-J2细胞,采用比色法测定分析细胞增殖变化；用实时荧光定量RT-PCR方法检测Zn T2、DMT1、IREG-1、MT1及ZIP4 mRNA表达,以TBPmRNA的表达水平作为内参对照.在细胞培养前36 h,乳酸锌对IPEC-J2细胞基本没有影响,随锌浓度递增细胞增殖幅度升高；乳酸锌处理IPEC-J2细胞后,Zn T2、DMT1、IREG-1及MT1 mRNA表达随锌浓度增高而升高,ZIP4 mRNA表达则随锌浓度增高而降低.添加乳酸锌可以促进IPEC-J2细胞增殖,上调ZnT2、DMT1、IREG-1、 MT1 mRNA表达,下调ZIP4 mRNA表达.

Effect of Zinc Lactate on Cell Proliferation and Related-regulatory Genes mRNA Expression in Porcine Jejunal Epithelial Cells IPEC-J2

The present study was conducted to evaluate the effects of zinc lactate on cell proliferation of jejunal epithelial cells IPEC-J2 and mRNA expression of related-regulatory genes in porcine.IPEC-J2 cells were cultured with mediums added with zinc lactate by the zinc concentrations of 50,100,150 and 200 mg·L-1,respectively.Cell proliferation was estimated by Colorimetric analysis.Simultaneously,real-time quantitative RT-PCR was applied to detect the mRNA expression of related-regulatory genes(ZnT2,DMT1,IREG-1,MT1 and ZIP4),and TBP mRNA level was used as the control.During the first 36 hours after treatment with zinc lactate,there was almost no effect on IPEC-J2 cell proliferation.Subsequently,with increasing of the concentration of zinc,the rate of cell proliferation gradually increased.At the same time,after IPEC-J2 cell was treated with zinc lactate,the expression of ZnT2,DMT1,IREG-1and MT1 mRNA was increased with the increase of the concentration of zinc while the expression of ZIP4 mRNA decreased.Zinc lactate could promote cell proliferation of IPEC-J2,up-regulated the mRNA expression of ZnT2,DMT1,IREG-1,MT1 and down-regulated the mRNA expression of ZIP4.