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| 青蒿琥酯纳米乳中青蒿琥酯的分光光度法和HPLC法分析 | |
| 引用本文： | 李均亮,李冰,周绪正,张继瑜. 青蒿琥酯纳米乳中青蒿琥酯的分光光度法和HPLC法分析[J]. 湖北农业科学, 2012, 51(8): 1678-1680 |
| 作者姓名： | 李均亮 李冰 周绪正 张继瑜 |
| 作者单位： | 中国农业科学院兰州畜牧与兽药研究所/农业部兽用药物创制重点实验室/甘肃省新兽药工程重点实验室,兰州,730050 |
| 基金项目： | 中央级科研院所基本科研业务费，国家现代农业肉牛牦牛产业技术体系建设专项资金，甘肃省生物技术项目 |
| 摘 要： | 比较了HPLC法和紫外分光光度法测定青蒿琥酯的含量,HPLC法和紫外分光光度法的线性范围分别为5～50μg/mL (R2=0.9992)和5～100 μg/mL (R2=0.9967),两种方法的分析结果无明显差异,表明这两种方法可较为准确地测定青蒿琥酯纳米乳中青蒿琥酯的含量,其中紫外分光光度法的操作简单,适合分析杂质含量低的样品,而HPLC法分辨率高,适用于成分复杂青蒿琥酯制剂的检测. |
| 关 键 词： | 高效液相色谱 分光光度法 青蒿琥酯 定量分析 |
Determination of Artesunate in Artesunate-Nanoemulsion by Spectrophotometry and HPLC |
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| LI Jun-liang , LI Bing , ZHOU Xu-zheng , ZHANG Ji-yu. Determination of Artesunate in Artesunate-Nanoemulsion by Spectrophotometry and HPLC[J]. Hubei Agricultural Sciences, 2012, 51(8): 1678-1680 | |
| Authors: | LI Jun-liang LI Bing ZHOU Xu-zheng ZHANG Ji-yu |
| Affiliation: | (Lanzhou Institute of Animal Science and Veterinary Pharmaceutics,Chinese Academy of Agriculture Sciences/Key Laboratory of New Animal Drug Project/Key Lab of Veterinary Pharmaceutics Discovery,Ministry of Agriculture,Lanzhou 730050,China) |
| Abstract: | The comparison of HPLC and spectrophotometry methods for detecting artesunate was carried out.The results showed that the linear rang of artemisinin was 5～50 μg/mL(R2=0.999 2) in HPLC,and 5～100 μg/mL(R2=0.996 7) in spectrophotometry.Both methods for determining the content in artesunate in artesunate-nanoemulsion showed no significant difference.Spectrophotometry was suitable for the detection of sample with low impurity,HPLC was used for complex sample. |
| Keywords: | HPLC spectrophotometry artesunate quantitative analysis |
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