黄秋葵纤维素合酶基因家族鉴定及表达分析

为了研究黄秋葵(Hibiscus esculentusL.)果实老化的机理,测定了其发育过程和采后纤维素含量,显微观察了纤维素在细胞结构中的分布.结果 表明,黄秋葵果实老化过程中纤维素含量及在细胞壁的分布大量增加,推测纤维素增加是果实老化的主导因素.从黄秋葵果实转录组测序的RNA-seq数据库中筛选得到9条功能注释为C...

Identification and Expression Analysis of CESA Gene Family in Hibiscus esculentus

In order to study the aging mechanism of Hibiscus esculentus fruit,the changes of cellulose content were measured during fruit development and postharvest period,and the distribution of cell structures were observed by microscope. The results showed that during the aging process of H. esculentus fruit,the content of cellulose increased greatly. It was suggested that the increase of cellulose was the main factor of fruit aging. Moreover,nine fragments annotated as CESA gene family were screened from the RNA-seq database of H. esculentus fruit,then the full-length cDNA sequences of CESA1,CESA2,CESA3,CESA4,CESA6,CESA7,CESA8 genes and CESA5 and CESA9 gene fragments were cloned. Expression of these nine genes of cellulose synthase gene family in H. esculentus was analyzed by fluorescence quantitative PCR. The results showed HeCESA1,He CESA3 and He CESA6 had the same expression pattern and conserved sequences CQIC and SVICEXWF motifs of primary cell wall specific CESAs. HeCESA4,HeCESA8 and HeCESA7 had the same expression pattern,and their expression levels were significantly up-regulated during fruit development and postharvest storage when cellulose increased significantly,which was much higher than that of other CESA genes at the same period. It can be inferred that HeCESA4,HeCESA8 and HeCESA7 play an important role in cellulose synthesis of H. esculentus fruit during senescence.