| 拟南芥HsfA2基因转化地被菊的研究 |
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| 引用本文： | 赵伶俐,石少川,孙佳琦,张启翔,高亦珂.拟南芥HsfA2基因转化地被菊的研究[J].北京林业大学学报,2011,33(5):97-102. |
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| 作者姓名： | 赵伶俐 石少川 孙佳琦 张启翔 高亦珂 |
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| 作者单位： | 北京林业大学园林学院，国家花卉工程技术研究中心 |
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| 基金项目： | “863”国家高技术研究发展计划项目(2008AA10Z156) |
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| 摘 要： | 利用农杆菌介导法将从哥伦比亚生态型拟南芥中克隆得到的热激转录因子A2基因导入地被菊‘北林黄’中,经抗生素筛选和分子检测,获得了8株阳性转化株系。利用RT-PCR方法对阳性转化株检测表明,其中7个株系中外源基因得到表达;高温胁迫处理结果也显示,转基因植株耐热胁迫能力显著高于非转基因植株。本文通过将外源AtHsfA2基因导...
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| 关 键 词： | AtHsfA2基因 遗传转化 地被菊 热胁迫 |
| 收稿时间： | 1900-01-01 |
Transformation of ground-cover Chrysanthemum with HsfA2 gene isolated from Arabidopsis thaliana |
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| ZHAO Ling-li,SHI Shao-chuan,SUN Jia-qi,ZHANG Qi-xiang,GAO Yi-ke.Transformation of ground-cover Chrysanthemum with HsfA2 gene isolated from Arabidopsis thaliana[J].Journal of Beijing Forestry University,2011,33(5):97-102. |
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| Authors: | ZHAO Ling-li SHI Shao-chuan SUN Jia-qi ZHANG Qi-xiang GAO Yi-ke |
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| Institution: | National Engineering Research Center for Floriculture，College of Landscape Architecture, Beijing Forestry University，100083，P. R. China. |
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| Abstract: | A HsfA2 gene isolated from Arabidopsis thaliana ecotype Columbia was transferred into ground-cover Chrysanthemum 'Beilinhuang' by the Agrobacterium-mediated method.After antibiotics screening and molecular detection,eight positive transgenic lines were obtained.RT-PCR detection results showed that the exogenous AtHsfA2 gene was expressed in seven transgenic lines.The results of heat stress treatment also showed that the ability of transgenic plants to heat stress was significantly higher than that of non-tr... |
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| Keywords: | AtHsfA2 gene genetic transformation ground-cover Chrysanthemum heat stress |
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