电化学发光猝灭法检测环孢霉素A |
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引用本文: | 江晓芬,钟茂生,梁剑,朱品玲,陈舒奕,邱彬.电化学发光猝灭法检测环孢霉素A[J].安徽农业科学,2013,41(4):1444-1445,1455. |
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作者姓名: | 江晓芬 钟茂生 梁剑 朱品玲 陈舒奕 邱彬 |
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作者单位: | 中华人民共和国宁德出入境检验检疫局,福建宁德,352100;福州大学,福建福州,350026 |
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摘 要: | 目的]建立用静态电化学发光分析法检测环孢霉素的方法。方法]以实验室自合成的羧基钌为发光试剂,环境友好型二丁基乙醇胺为共反应试剂,用静态电化学发光分析法检测环孢霉素。结果]在pH为6.0的0.06 mol/L PBS缓冲溶液中,羧基钌发光强度的淬灭值与环孢霉素A浓度在9.3×10-9~4.0×10-7mol/L之间呈良好的线性关系(R2=0.995 0),检测限达5.4×10-7mol/L。结论]该方法是首次用电化学发光方法应用于环孢霉素的检测,为电化学发光方法检测环孢霉素提供了理论与试验依据。
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关 键 词: | 电化学发光 羧基钌 环孢霉素A |
Determination of Cyclosporine A by Electrochemiluminescent Method |
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Institution: | JIANG Xiao-fen et al (Ningde Import and Export Inspection and Quarantine Bureau, Ningde, Fujian 352100) |
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Abstract: | Objective ] A static electroehemiluminescent approach was developed to determine cyclosporine A. Method] By using the syn- thetic carboxyl ruthenium as luminescent reagents and environmental friendly dibutyl ethanolamine as the co-reaction reagent, cyclosporine was determined by static electrochemiluminescence analysis. Resuh ] Linear responses in chemiluminescence quenching of carboxyl ruthenium and concentration of cyclosporine A were good linearity (R2 = 0.995 0) between 9.3x10^-9 mol/L and 4.0 x 10 ^-7 mol/L in 0.06 mol/L PBS buffer solution ( pH 6.0). The corresponding limit of detection (LOD) was 5.4 x 10 ^-7 mol/L. Conclusion] The method is suitable for the determination of cyclosporine by electrochemiluminescence for the first time and provides theoretical and experimental supports |
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Keywords: | Electrochemiluminescent Carboxyl ruthenium Cyclospofine A |
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