48 份分蘖洋葱种质资源遗传多样性的ISSR 标记和农艺性状分析

对来自黑龙江省和吉林省不同地区的48 份分蘖洋葱资源的遗传多样性进行了ISSR 标记及农
艺性状观测分析。从51 条ISSR 引物中筛选到13 条多态性高、扩增稳定、重复性好的引物，这13 条引
物共扩增出132 个位点，其中109 个多态位点；应用Nei-Li 相似系数法估算了48 份材料间的遗传相似系
数，结果表明，48 份分蘖洋葱遗传相似系数变化范围在0.59 ～ 0.94 之间。对分蘖洋葱的抽薹情况、幼叶
色、鳞茎色和单球质量进行了调查，结果表明，48 份分蘖洋葱遗传相似系数变化范围在0.38 ～ 1.00 之间。
ISSR 标记和农艺性状分析均表明48 份分蘖洋葱的遗传多样性较丰富；两种方法的聚类结果基本一致，聚
类结果与地理分布有明显的联系。

ISSR Markers of 48 Tillering Onion(Allium cepa var.aggregatum) Germplasms Genetic Diversity and Analysis of Their Agronomic Traits

Inter-simple sequence repeat（ ISSR） markers and agronomic traits were analyzed to
observe the genetic diversity of 48 tillering onion（Allium cepa var. aggregatum）germplasms from
Heilongjiang and Jilin Provinces in China．Thirteen ISSR primers with rich polymorphism，stable amplification
and good repeatability were screened from 51 ISSR primers．With these screened primers，
132 bands were identified，of which 109 bands were polymorphic．The genetic similarity was estimated
with Nei-Li coefficient，and the results showed that the genetic similarity coefficients of 48 tillering
onion germplasms ranged from 0.59 to 0.94．The investigation was also carried out on their agronomic
traits，including bolting，leaf color，seed coat color，and single fruit quality．The results showed that
the genetic similarity coefficients of these 48 tillering onion germplasms ranged from 0.38 to 1.00．Both
results indicated that the genetic diversity of 48 tillering onion germplasms was rich．The clustering results
of these 2 methods were basically identical，and the clustering results had obvious connection with
their geographic origins.