Expression of myeloperoxidase in swine influenza virus (SIV)-infected neutrophils in lungs from pigs experimentally infected with SIV subtype H1N2 |
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Authors: | Bongtae Kim Jeoung Hwa Shin Kiwon Han Hwi Won Seo Yeonsu Oh Ikjae Kang Changhoon Park Bog-Hieu Lee Jin Sil Jang Sung-Hoon Kim Chanhee Chae |
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Affiliation: | Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 599 Gwanak-ro, Gwanak-Gu, 151-742, Seoul, Republic of Korea. |
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Abstract: | The expression of myeloperoxidase (MPO) was examined in the swine influenza virus (SIV)-infected neutrophils in the lungs of pigs experimentally infected with swine influenza virus (SIV) subtype H1N2 by immunohistochemistry. Five pigs each from the infected and non-infected group were euthanized 1, 3, 5, 7, and 10?days post-inoculation (dpi). Immunohistochemical reactivity was mainly seen in neutrophils. The score for pulmonary histopathological lesions correlated with the score for MPO immunohistochemical reactivity (r ( s )?=?0.962, P?0.01). In addition, the score for in situ hybridization of SIV nucleic acid correlated with the score for MPO immunohistochemical reactivity (r ( s )?=?0.976, P?0.01). These results suggest neutrophils are one of the primary effector cells in the early phase of SIV infection in pigs. |
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