| 白菜黑斑病拮抗细菌8-59的筛选及鉴定 |
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| 引用本文： | 邢祎博,王全,李术娜,李朝玉,李红亚,雷白时,王树香,朱宝成. 白菜黑斑病拮抗细菌8-59的筛选及鉴定[J]. 安徽农业科学, 2010, 38(16): 8495-8497,8562 |
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| 作者姓名： | 邢祎博 王全 李术娜 李朝玉 李红亚 雷白时 王树香 朱宝成 |
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| 作者单位： | 河北农业大学生命科学学院,河北保定,071001 |
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| 基金项目： | 河北省自然科学基金项目 |
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| 摘 要： | [目的]筛选对白菜黑斑病病原菌具有拮抗作用的细菌。[方法]从各地的土壤中分离到200多株拮抗细菌,进行初筛、复筛,再对筛选到的菌株进行菌体形态、菌落特征的观察及一系列生理生化试验。[结果]200多株拮抗细菌中菌株8-59有显著的抑菌活性,根据8-59菌株的形态特征和生理生化特征综合考察,初步判断其为巨大芽孢杆菌（Bacillus megaterium）。[结论]该研究可为细菌拮抗机理研究及后续的生产与田间应用奠定基础。
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| 关 键 词： | 白菜黑斑病 拮抗细菌 初筛 复筛 鉴定 |
Isolation and Identification of Antagonistic Bacterium Strain 8-59 against Alternaria brassicae |
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| XING Yi-bo et al. Isolation and Identification of Antagonistic Bacterium Strain 8-59 against Alternaria brassicae[J]. Journal of Anhui Agricultural Sciences, 2010, 38(16): 8495-8497,8562 |
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| Authors: | XING Yi-bo et al |
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| Affiliation: | XING Yi-bo et al(College of Life Science,Agricultural University of Hebei,Baoding,Hebei 071001) |
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| Abstract: | [Objective]The research aimed to screen antagonistic bacterium to Alternaria brassicae.[Method]More than 200 antagonistic strains were isolated from soil of different districts,and their antagonism to A.brassicae were tested through primary screening and secondary screening.Than mycelium shape and colony characteristic observation,and a series of physiological and biochemistry experiments had been done on the selected strain.[Result]The results showed that 8-59 strain had strong inhibition capability agains... |
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| Keywords: | Alternaria brassicae Antagonistic bacterium Primary screening Secondary screening Identification |
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