Expression,Characterization and Antimicrobial Ability of a Variant T4 Lysozyme in Pichia pastoris |
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| Authors: | Ning SUN Sanfeng CHEN Xiangming XIE Yueju WANG Gangqiang LI Nan WANG DehuLIU |
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| Institution: | [1]Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China [2] Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China [3] Department of Microbiology, Col ege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China [4] Biology Department, Macon State Col ege, University System of Georgia, Macon, Georgia 31206, USA |
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| Abstract: | T4 lysozyme was engineered with disulfide bonds and expressed in Pichia pastoris. The secreted proteins were purified and made into powder by lyophilization. Recombinant protein purity was more than 70% measured by HPLC. The lytic activity of variant T4-lysozyme was measured by the lysis of the cell wall of Xanthomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, Ralstonia solanacearum comb.nov, Clavibacter michiganensis subsp. michiganensis, X. campestris pv. malvacearum, Fusarium oxysporium sp. vasinfectum, Verticillium dahliae kleb. Inhibition zone assay showed that variant T4 lysozyme significantly inhibited X. o. oryzicola and X. c. malvacearum. The antifungal activities of this protein against F. o. vasinfectum and V. d. kleb were also analyzed. |
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| Keywords: | Antibacterial activity Expression Pichia pastoris T4 lysozyme Variant |
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