| 牛流行热病毒山东地方株的分离与鉴定 |
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| 引用本文： | 王洪梅,侯佩莉,杨宏军,宋玲玲,何洪彬.牛流行热病毒山东地方株的分离与鉴定[J].山东农业科学,2013,45(2):31-33,37. |
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| 作者姓名： | 王洪梅 侯佩莉 杨宏军 宋玲玲 何洪彬 |
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| 作者单位： | 山东省农业科学院奶牛研究中心,山东济南,250131 |
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| 基金项目： | 山东省自然科学基金项目(ZR2010CM012);现代农业(奶牛)产业技术体系项目;泰山学者海外特聘专家项目;国家自然科学基金项目(31072160);济南市高校院所主创新计划项目(201004027,201202059,201102034);山东省科技攻关课题(2009GG20002032);山东省青年自然科学基金项目(ZR2010ZR029) |
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| 摘 要： | 利用BHK-21细胞,接种山东济南地区疑似牛流行热病牛高热期的抗凝血,经盲传3代,分离到1株病毒,经RT-PCR鉴定为牛流行热病毒,命名为BEFV-SD。理化特性试验表明:BEFV-SD对热敏感,56℃10 min、37℃18 h可被灭活,对乙醚、氯仿等敏感;BEFV-SD可被BEFV阳性血清中和,中和效价为1∶408;将BEFV-SD的G基因与GenBank中公布的BEFV进行同源性比较,同源性达到96.3%以上。
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| 关 键 词： | 牛流行热病毒 分离 鉴定 同源性 |
Isolation and Identification of Bovine Ephemeral Fever Virus Strain in Shandong Province |
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| Wang HongMei
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Hou PeiLi
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Yang HongJun
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Song LingLing
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He HongBin.Isolation and Identification of Bovine Ephemeral Fever Virus Strain in Shandong Province[J].Shandong Agricultural Sciences,2013,45(2):31-33,37. |
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| Authors: | Wang HongMei Hou PeiLi Yang HongJun Song LingLing He HongBin |
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| Institution: | *(Research Center of Dairy Cattle,Shandong Academy of Agricultural Sciences,Jinan 250131,China) |
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| Abstract: | One virus strain named as BEFV-SD was isolated from the infected cattle in Shandong Province,and it was systematically identified by a series of methods through cultivated in BHK-21 cells.In result,the isolate could cause typical cytopathogenic effect in the BHK-21 cells after infection.The BEFV-SD could be neutralized by the positive sera with bovine ephemeral fever virus at the titer of 1∶ 408.The isolate virus was sensitive to chloroform,diethyl ether and heat,and could be inactivated at 56℃ for 10 minutes or 37℃ for 18 hours.The G gene was amplified by RT-PCR from the BHK-21 cells infected with BEFV-SD virus.The homology of G gene nucleic acid sequences among the virus in GenBank was more than 96.3%. |
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| Keywords: | Bovine ephemeral fever virus Isolation Identification Homology |
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