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| 鲫外周血淋巴细胞的分离及观察 | |
| 引用本文： | 秦玉丽,汪成竹. 鲫外周血淋巴细胞的分离及观察[J]. 河南农业科学, 2008, 0(6) |
| 作者姓名： | 秦玉丽 汪成竹 |
| 作者单位： | 信阳农业高等专科学校,河南,信阳,464000 |
| 摘 要： | 采用密度为1.085g/mL的聚蔗糖-泛影葡胺(Ficoll-hypaque)分离液在20℃,800g,离心20min的情况下,可将鲫外周血中的淋巴细胞有效地分离出来,每毫升外周血可分离到的淋巴细胞数量平均为4.88×106个,淋巴细胞回收率为76.21%～93.79%,细胞存活率≥96%。 |
| 关 键 词： | 鲫 淋巴细胞 分离 观察 |
Separation and Observation of Lymphocyte of Carassius auratus |
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| QIN Yu-li,WANG Cheng-zhu. Separation and Observation of Lymphocyte of Carassius auratus[J]. Journal of Henan Agricultural Sciences, 2008, 0(6) | |
| Authors: | QIN Yu-li WANG Cheng-zhu |
| Abstract: | The lymphocyte of carp peripheral blood could be separated by the density 1.085g/mL Ficoll-hypaque at 20 ℃ and 800 g centrifugation for 20 min.The average number of lymphocyte cells was 4.88 × 106 in one milliliter of blood,and the rate of lymphocyte recovery was 76.21%-93.79%.The cell survival rate was greater than or equal to 96%. |
| Keywords: | Carassius auratus Lymphocyte Separation Observation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |