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草莓果实维生素C提取的影响因素研究
引用本文:高媛,张丙秀,高庆玉,代志国,张志昆.草莓果实维生素C提取的影响因素研究[J].河南农业科学,2013,42(4).
作者姓名:高媛  张丙秀  高庆玉  代志国  张志昆
作者单位:1. 东北农业大学园艺学院,黑龙江哈尔滨150030;农业部东北地区园艺作物生物学与种质创制重点实验室,黑龙江哈尔滨150030
2. 农业部东北地区园艺作物生物学与种质创制重点实验室,黑龙江哈尔滨,150030
基金项目:农业部公益性行业(农业)科研专项,东北农业大学博士启动基金
摘    要:为了找出提取草莓维生素C更高效的方法,以紫外分光光度法测定其含量,探讨了提取液、蔗糖、反应时间对草莓维生素C提取量的影响。结果表明:在三氯乙酸为提取液、Cu2+溶液中含有13.75g/L蔗糖的条件下,当温度30℃、温热时间30min时,还原型维生素C(AA)的回收率最高(88%),在还原温度30℃、还原时间30min时,氧化型维生素C(DHAA)的回收率最高(81%)。

关 键 词:草莓  维生素C  紫外分光光度法

Influencing Factors on Extraction of Vitamin C from Strawberry
GAO Yuan , ZHANG Bing-xiu , GAO Qing-yu , DAI Zhi-guo , ZHANG Zhi-kun.Influencing Factors on Extraction of Vitamin C from Strawberry[J].Journal of Henan Agricultural Sciences,2013,42(4).
Authors:GAO Yuan  ZHANG Bing-xiu  GAO Qing-yu  DAI Zhi-guo  ZHANG Zhi-kun
Abstract:The vitamin C content of strawberry was determined by UV spectrophotometry,and the effects of extraction solutions,sucrose and response time on extraction of vitamin C from strawberry were studied.The results showed that when trichloroacetic acid was used as extraction solution,Cu2+ solution contained 13.75 g/L sucrose,the temperature was 30 ℃,the heat time was 30 min,the recovery rate of reduced vitamin C(AA) was highest(88%);when the reduction temperature was 30 ℃ and reduction time was 30 min,the oxidized vitamin C(DHAA) recovery was highest(81%).
Keywords:strawberry  vitamin C  UV spectrophotometry
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