链轮丝菌属谷氨酰胺转氨酶的研究进展

谷氨酰胺转氨酶能催化酰基转移反应,使蛋白质分子间和分子内产生共价交联,从而改变蛋白质的功能与特性。与其他来源的谷氨酰胺转氨酶相比,微生物谷氨酰胺转氨酶因其不需要钙离子激活、分子量小、易于纯化、应用成本低等特性,在食品、医药等领域显示出更广阔的应用前景。微生物谷氨酰胺转氨酶主要来源于链轮丝菌属。 本文对链轮丝菌属产谷氨酰胺转氨酶菌株的选育、发酵条件的优化、MTG的分离纯化、MTG结构、MTG基因的克隆、转化和修饰、MTG的应用等方面进行了综述。

Research Advance in Transglutaminase in Streptoverticillium

To illuminate the application prospects of transglutaminase in Streptoverticillium in the fields of food and medicine, the authors summarized catalytic activity, physiological function and physicochemical properties of transglutaminase; summed up the breeding of high- yield transglutaminase strain, the optimization of fermentation conditions of producing transglutaminase strain, isolation and purification of transglutaminase in Streptoverticillium, gene cloning of transglutaminase, sequence analysis and transformation, gene modification, the structure and application of transglutaminase in Streptoverticillium. The problems of transglutaminase in the commercial production were pointed out, and the future research hotspots and development trend of transglutaminase in Streptoverticillium were discussed.